Anti-GABA-A-R-Alpha6 Antibody (N229A/32)

Name: Anti-GABA-A-R-Alpha6 Antibody
Brand: NeuroMab
SKU: 75-388
Price: 319.0 USD
Availability: InStock

3 Citations

Our Anti-GABA-A-R-Alpha6 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N229A/32. It detects mouse and rat GABA-A-R-Alpha6, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against extracts of COS cells transiently transfected with DsRed-tagged GABAARAlpha4, Flag-tagged GABAARAlpha6 or untagged Kv2.1 plasmid probed with N229A/32 (left), Mouse anti-Flag (middle) or N398A/34 (right) TC supe.

Adult rat brain immunohistochemistry.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-388

Product Details

Target

GABA-A-R-Alpha6

Target Description

Gamma-aminobutyric acid receptor subunit alpha-6 or GABA-A-R-Alpha6 is encoded by the gene GABRA6. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-Alpha6 is primarily expressed in brain at the cell junction, synapse, the postsynaptic cell membrane and cytoplasmic vesicle membrane. Diseases associated with GABRA6 include neurotic disorder and several forms of epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N229A/32

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Gabra6 Gabra-6

Molecular Weight

50 kDa

Antigen

Fusion protein amino acids 325-420 (last cytoplasmic domain before extracellular C-terminus) of mouse GABA-A-R-Alpha6 (accession number P16305) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2491091

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Does not react with GABA-A-R-Alpha4

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit alpha-6 (GABA(A) receptor subunit alpha-6)

UniProt Details

UniProt (Human): Q16445
UniProt (Immunogen Species): P16305

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
37108794	not listed	Chen, M., et al. 2023. Blue Native PAGE-Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype. International Journal of Molecular Sciences, 7632.
36775097	1:500	Gruol, D.L., et al. 2023. Neuroimmune Interactions with Binge Alcohol Drinking in the Cerebellum of IL-6 Transgenic Mice. Neuropharmacology, 109455.
32634532	1:500	Gruol, D.L., et al. 2020. Alcohol and IL-6 Alter Expression of Synaptic Proteins in Cerebellum of Transgenic Mice With Increased Astrocyte Expression of IL-6. Neuroscience, 124-137.

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Anti-GABA-A-R-Alpha6 Antibody (N229A/32)

SKU: 75-388

Product Details

Product Specific References for Applications and Species

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