Anti-Neuroligin-1 Antibody (N97A/31)

31 Citations

Our Anti-Neuroligin-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N97A/31. It is KO validated, detects mouse and rat Neuroligin-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.
Immunoblot against crude membranes from adult mouse brain (MBM), extracts of COS cells transiently transfected with Neuroligin-1, -2, -3, -4 or -4* plasmid and extracts of brains from wild-type (WT) and Neuroligin-1 knockout (KO) mice probed with N97A/31 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof (Stanford).
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N97A/31 (left) or N52A/42 (right) TC supe.
Confocal microscopy analysis of NLGN1(red) and APC (magenta) co-staining in HuTu 80 cells. Image from publication CC-BY-4.0. PMID:36056393
Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.
Immunoblot against crude membranes from adult mouse brain (MBM), extracts of COS cells transiently transfected with Neuroligin-1, -2, -3, -4 or -4* plasmid and extracts of brains from wild-type (WT) and Neuroligin-1 knockout (KO) mice probed with N97A/31 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof (Stanford).
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N97A/31 (left) or N52A/42 (right) TC supe.
Confocal microscopy analysis of NLGN1(red) and APC (magenta) co-staining in HuTu 80 cells. Image from publication CC-BY-4.0. PMID:36056393

Chicken, Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650

Go to Full Product Details

Data Sheet
KO Validated KO Validated

SKU: 75-160

Volume: 100 µL
1-2 business days
Price:
$319.00

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
32434929not listedKim, H., et al. 2020. Calsyntenin-3 interacts with both α-and β-neurexins in the regulation of excitatory synaptic innervation in specific Schaffer collateral pathways. Journal of Biological Chemistry, 9244-9262.
22355283not listedYamagata, M., et al. 2012. Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP).. Frontiers in Molecular Neuroscience, 18.
Immunocytochemistry: Mouse
PMID Dilution Publication
360075211:1000Nozawa, K., et al. 2022. In vivo nanoscopic landscape of neurexin ligands underlying anterograde synapse specification. Neuron, 3168-3185.
3308376920ug/mlVerstraelen, P., et al. 2020. Systematic quantification of synapses in primary neuronal culture. iScience, 101542.
29760652not listedYamagata, M., et al. 2018. Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation.. Frontiers in Molecular Neuroscience, 142.
Immunohistochemistry: Chicken
PMID Dilution Publication
210315601:500Wahlin KJ, et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62.
Immunohistochemistry: Human
PMID Dilution Publication
360563931:100Pergolizzi, M., et al. 2022. The Neuronal Protein Neuroligin 1 Promotes Colorectal Cancer Progression by Modulating the APC/β-catenin Pathway. Journal of Experimental & Clinical Cancer Research, 266.
Immunohistochemistry: Mouse
PMID Dilution Publication
253527481:400de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-33.
Western Blot: Chicken
PMID Dilution Publication
210315601:500Wahlin KJ, et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62.
Western Blot: Human
PMID Dilution Publication
32434929not listedKim, H., et al. 2020. Calsyntenin-3 interacts with both α-and β-neurexins in the regulation of excitatory synaptic innervation in specific Schaffer collateral pathways. Journal of Biological Chemistry, 9244-9262.
Western Blot: Mouse
PMID Dilution Publication
384726491:1000Campos, J., et al. 2024. Liprin-α proteins are master regulators of human presynapse assembly. Nature Neuroscience, .
373080471:800Hernandez, A, et al. 2023. Striatal Synaptic Changes and Behavior in Adult Mouse Upon Prenatal Exposure to Valproic Acid. Brain Research, 148461.
372607471:1000Arias-Aragón, F, et al. 2023. A Neuroligin-1 Mutation Associated with Alzheimer's Disease Produces Memory and Age-dependent Impairments in Hippocampal Plasticity. iScience, 106868.
370784491:3000Yokokawa, T., et al. 2023. Altered Expression of Synaptic Proteins and Adhesion Molecules in the Hippocampus and Cortex Following the Onset of Diabetes in Nonobese Diabetic Mice. Physiological Reports, e15673.
330837691ug/mlVerstraelen, P., et al. 2020. Systematic quantification of synapses in primary neuronal culture. iScience, 101542.
288648261:300Tanabe, Y., et al. 2017. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α. Nature communications, 408.
270059901:10000Heise, C., et al. 2017. eEF2K/eEF2 pathway controls the excitation/inhibition balance and susceptibility to epileptic seizures. Cerebral Cortex, 27(3), pp.2226-2248.. Cerebral Cortex, 2226-2248.
268598201:2000Kim, J.Y.V., et al. 2016. Neuroligin 2 regulates spinal GABAergic plasticity in hyperalgesic priming, a model of the transition from acute to chronic pain. Pain, 1314-1324.
26776509not listedUm, J.W., et al. 2016. LRRTM3 regulates excitatory synapse development through alternative splicing and neurexin binding. Cell Reports, 808-822.
26094033not listedZou, D., et al. 2015. A critical role of RBM8a in proliferation and differentiation of embryonic neural progenitors.. Neural Development, 18.
247955611:1000Lugo, J.N., et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27.
24448406not listedLi Q, et al. 2014. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.. Elife, e01201.
237198051 : 1000Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384.
211450031:1000Robbins EM, et al. 2010. SynCAM 1 adhesion dynamically regulates synapse number and impacts plasticity and learning.. Neuron, 894-906.
Western Blot: Rat
PMID Dilution Publication
32801157not listedCulotta, L., et al. 2020. SULT4A1 modulates synaptic development and function by promoting the formation of PSD-95/NMDAR complex. Journal of Neuroscience, 7013-7026.
283628573.3ug/mlBurch, A., et al. 2017. A novel synaptic junction preparation for the identification and characterization of cleft proteins.. PLoS One, e0174895.
278698011:1000Vieweg, S., et al. 2017. Direct dorsal hippocampal–prelimbic cortex connections strengthen fear memories. Nature neuroscience, 20(1), pp.52-61.. Nature neuroscience, 52-61.
276778491:200Hanus, C., et al. 2016. Unconventional secretory processing diversifies neuronal ion channel properties. Elife, e20909.
27002143not listedUm, J.W., et al. 2016. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation.. The Journal of Biological Chemistry, 10119-10130.
Additional Publications: Unspecified
PMID Publication
30602588Binda, C.S., et al. 2019. Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number. The Biochemical Journal, 293-306.
30046996Nozawa, K., et al. 2018. Cellular and Subcellular Localization of Endogenous Neuroligin-1 in the Cerebellum. Cerebellum, 709-721.

Related Products

Recently Viewed