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Figure 1. Structure of GlutathioneFigure 2. Glutathione Reactions
ImmunoChemistry Technologies Glutathione Colorimetric Detection Kit
$442
Glutathione Fluorescent Detection KitGlutathione Fluorescent Detection Kit
ImmunoChemistry Technologies Glutathione Fluorescent Detection Kit
From $427.50
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ImmunoChemistry Technologies Glycerol Fluoro Mount with PPD
From $80.75
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ImmunoChemistry Technologies Glycerol Mounting Medium
From $74.50
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ImmunoChemistry Technologies Glycerol Mounting Medium with DABCO
From $85.75
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ImmunoChemistry Technologies Glycerol Mounting Medium with DAPI and DABCO
From $97.25
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Aves Labs Goat Anti-Chicken IgY Secondary Antibody
$120
Antibodies Incorporated Goat Anti-Guinea Pig IgG Secondary Antibody
From $95
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Antibodies Incorporated Goat Anti-Hamster Gamma Globulins Secondary Antibody
From $105
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Antibodies Incorporated Goat Anti-Hamster IgG Secondary Antibody
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Antibodies Incorporated Goat Anti-Human Gamma Globulins Secondary Antibody
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Antibodies Incorporated Goat Anti-Human IgG Secondary Antibody
From $80
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Antibodies Incorporated Goat Anti-Monkey (Rhesus) IgG Secondary Antibody
From $90
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Antibodies Incorporated Goat Anti-Mouse IgG Secondary Antibody
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Antibodies Incorporated Goat Anti-Rabbit Gamma Globulins Secondary Antibody
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Antibodies Incorporated Goat Anti-Rabbit IgG Secondary Antibody
From $105
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Antibodies Incorporated Goat Anti-Rat IgG Secondary Antibody
From $105
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Figure 1. R110-(RR)2 was reconstituted in DMSO, diluted in an aqueous buffer, and then spiked with cathepsin B enzyme and incubated at 37°C for 1 hour. After incubation, fluorescence was analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.Figure 2. Jurkat cells were treated with the cathepsin inhibitors CA-074 Me (blue) and E-64d (gray), or were untreated (green) and then stained with R110-(RR)2. Samples were then analyzed analyzed by flow cytometry (488 nm excitation and a 530/30 emission filter). Treatment with CA-074 Me or E-64d decreased the fluorescence signal detected compared to the untreated control, which displayed a baseline level of cathepsin activity. Data courtesy of Dr. Kristi Strandberg (ICT 231:40-42).
ImmunoChemistry Technologies Green Fluorescent Cathepsin B Assay
From $206
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Figure 1. Monocytes can secrete active caspase-1, therefore cells were labeled with FAM-FLICA® Caspase-1 (WEHD) (cat. 9161/9162) prior to treatment. Cells were then treated with DMSO (control) or 10 µM nigericin (cat. 6698), stained with Hoechst 33342, fixed (cat. 636), and viewed using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Increased levels of FAM-FLICA® staining were seen in the nigericin-treated cells. Data courtesy of Dr. Kristi Strandberg, ICT (235:60).
ImmunoChemistry Technologies Green Fluorescent FAM FLICA® Caspase-1 (WEHD) Assay Kit
From $234
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Figure 1. Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.Figure 2. Staurosporine (cat. 6212) was injected into the forebrain of a female house sparrow to induce apoptosis. Approximately 20 hours later, FAM in vivo probe (cat. 980/981) was intravenously injected into the jugular and allowed to circulate 30 minutes. The bird was sacrificed and frozen brain sections prepared. Neurons with active caspases fluoresce green. This image shows one apoptotic neuron at 100X. Data courtesy of Mr. Chris Thompson at the University of Washington, Seattle.
ImmunoChemistry Technologies Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay
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Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, and then labeled with FAM-DEVD-OPH (cat. 6356). Wet-mount slides were prepared and green fluorescence (right) was detected using a band pass filter (excitation at 488 nm, emission at 520 nm). FAM-DEVD-OPH reveals complete caspase-3/7 activation in the form of green fluorescence in all cells. The corresponding DIC image is shown (left). Data courtesy of Ms. Tracy Hanson, ICT (ICT111709; FAM-DEVD-OPH_in_59_60).
ImmunoChemistry Technologies Green Fluorescent FAM-DEVD-OPH in vitro caspase-3/7 Apoptosis Detection Reagent
$555
Figure 1. THP-1 cells were treated with either a negative control (A), or PMA (5 ng/mL) followed by LPS (10 ng/mL). Cells were then stained with FAM-YVAD-FMK (cat. 97/98) and analyzed by phase contrast and fluorescence microscopy. In the treated sample, many cells appear bright green, indicating increased caspase-1 activity (B). In the non-induced sample, few green cells are visible, indicating a low level of caspase-1 activity (A). Data courtesy of Dr. Brian Lee, ICT (207:11).
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-1 (YVAD) Assay Kit
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Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay KitGreen Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
From $216
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Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay KitGreen Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
From $216
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