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Figure 1. Cell death in primary rat hippocampal neurons. (A) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (D). DNA is labeled in 4 cells (D), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.

Figure 2. Normal (A) and keratoconus (B) corneal fibroblasts were treated with 200 μM H2O2 for 1 hour, washed, and allowed to recover. Cells were then treated with FAM-DEVD-FMK (cat. 93/94) for 1 hour, then washed with 1X Apoptosis Wash Buffer. Keratoconus corneal fibroblasts treated with H2O2 (B) show a significant increase in caspase-3/7 activity compared to normal cells (A). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, University of California, Irvine.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $216.25

Figure 1. Caspase activities in the microglia of white matter injury mice using our FAM-FLICA® kits. Only caspase 1 was significantly upregulated. Figure 1 (d) of L. He et al (2022) miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury, Hindaw Journal of Immunology Research 2022, 1642896. https://doi.org/10.1155/2022/1642896. Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 Liufang He et al.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-6 (VEID) Assay Kit

From $216.25

Figure 1. Activity of caspase-8 (using FAM-LETD-FMK) in AGS cells after 24 h incubation with Les-4367 (1 μM) and combined with anti-HER2 antibodies. From Figure 4 of A. Gornowicz et al Multi-Targeting Anticancer Activity of a New 4-Thiazolidinone Derivative with Anti-HER2 Antibodies in Human AGS Gastric Cancer Cells, Int J Mol Sci (2023) 24, 6791. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-8 (LETD) Assay Kit

From $216.25

Figure 1. Flow cytometry analysis of active caspase-9 in MDA-MB-231 (A,B) and ZR-75-1 (C,D) cells. Cells were stained with FAM-FLICA® Caspase-9 reagent. From Figure 5 of R. Krętowski and M. Cechowska-Pasko, The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells, Int J Mol Sci (2022) 23, 9285. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 by the authors.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).

ImmunoChemistry Technologies Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit

From $206

ImmunoChemistry Technologies Green Fluorescent FAM-Leu-DAP Serine Protease Assay Kit

From $206

Figure 1. Jurkat cells were treated with staurosporine for 1, 2, 3, and 4 hours to induce apoptosis and increase serine protease activity (green), or were untreated (gray). Samples were stained with FAM-Phe-CMK (Kit 945/946) for 1 hour at 37°C, washed, and then analyzed using a flow cytometer. The amount of serine protease activity detected positively correlated to the duration of the exposure period. Data courtesy of Dr. Kristi Strandberg (ICT 231:75-79).

Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis and increase serine protease activity (upper), or left untreated (lower), then stained with FAM-Phe-CMK (Cat. 945/946), 660-VAD-FMK (cat. 9120), and Hoechst 33342. Imaged using EGFP (Ex 470/30, Em 530/50), Cy5 (Ex 620/60, Em 700/75), and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Staurosporine increased the intracellular levels of both serine proteases and caspases. Data courtesy of Dr. K. Strandberg (ICT 231:73).

ImmunoChemistry Technologies Green Fluorescent FAM-Phe-CMK Serine Protease Assay Kit

From $206

ImmunoChemistry Technologies Green Fluorescent FAM-Phe-DAP Serine Protease Assay Kit

From $212

ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit

From $206

ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit

From $206

Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, then stained with FAM- VAD-OPH I (Cat. 6354) or FAM-VAD-FMK FLICA® (Cat. 92), washed and then read on a flow cytometer. Results with the OPH inhibitor (grey solid) are consistent with those achieved using the FMK inhibitor (green outline). FAM-VAD-OPH inhibitors mirror FAM-VAD-FMK analogs for apoptosis detection, and they may be less toxic and more stable. Data courtesy of Dr. M. Olin, University of Minnesota (ICT-202).

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH I in vitro poly Caspase Apoptosis Detection Reagent

$555

Figure 1. Jurkat cells were treated with DMSO (left) or staurosporine (right), labeled with FAM-VAD-OPH II, washed, and stained with PI. Four cell populations are seen: unstained live cells do not fluoresce (lower left); early apoptotic cells fluoresce green with FAM- VAD-OPH II (lower right); late apoptotic cells (upper right) fluoresce green (active caspases) and red (permeabilized cell membrane); and necrotic cells fluoresce red (upper left). Data of Dr. M. Olin, University of Minnesota.

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH II in vitro poly Caspase Apoptosis Detection Reagent

$555

Figure 1. Fluorescent Microscopy Analysis of THP-1 Suspension Cells THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

Figure 2. Caspase Activity in Jurkat Cells Jurkat cells (T lymphocytes) were labeled with our Green Fluorescent Poly (active) Caspase FAM FLICA® Inhibitor Reagent (FAM-VAD-FMK) (catalog 92) and viewed under a fluorescence microscope. The grey DIC image (B) reveals five cells in the field of view, but only four of them fluoresce green (A). Four out of five cells are apoptotic and have active caspases present (green). Data courtesy of Dr. Brian W. Lee, ICT.

ImmunoChemistry Technologies Green Fluorescent Poly (active) Caspase FAM FLICA® Assay Kit (FAM-VAD-FMK)

From $216.25

Figure 2. Jurkat cells were exposed to 3% formaldehyde for 30 minutes. Following the formaldhehyde treatment, cells were stained with Green Live/ Dead stain and then imaged with a Nikon E800 microscope (DIC overlay shown). Cells with compromised membranes stained green, while cells with intact membranes excluded the Green Live/Dead stain and remained unstained. Data courtesy of Mrs. Tracy Murphy, ICT.

ImmunoChemistry Technologies Green Live/Dead Stain

$102.50

ImmunoChemistry Technologies Hematoxylin, Immuno/Histo Aqueous

From $58

ImmunoChemistry Technologies Hematoxylin, PROBE

$91

Figure 2. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer and seeded in glass bottom dishes. The cells were then loaded with APF (10μM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters

ImmunoChemistry Technologies Highly Reactive Oxygen Species (hROS) Detection Kit

$289

Figure 1. MR-(DEVD)2 (cat. 936) and Hoechst 33342 were used to quantify apoptosis in human PASMCs. Cells were treated with a negative control (A), a drug that inhibits proliferation (B), or H2O2 to induce apoptosis (C). Apoptotic cells stained red with cleaved MR-(DEVD)2 and blue with Hoechst. They have less intense blue nuclei than healthy cells, which have bright blue nuclei. Research of Dr. F. Perros, et al., Universite Paris-Sud 11; Clamart; Hopital Henri-Mondor, Creteil, France.

Figure 2. THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

ImmunoChemistry Technologies Hoechst 33342 Fluorescent Nucleic Acid Stain

$22.75

Aves Labs Horseradish Peroxidase Chicken Anti-Goat IgG Secondary Antibody

$165

Aves Labs Horseradish Peroxidase Chicken Anti-Mouse IgG Secondary Antibody

$165

Aves Labs Horseradish Peroxidase Chicken Anti-Rabbit IgG Secondary Antibody

$165

Aves Labs Horseradish Peroxidase Goat Anti-Chicken IgY Secondary Antibody

$165

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Mammalian, 1X

From $68.25

Figure 1. Stabilization of HRP conjugates

Figure 2. Minimizes background in mono-poly sandwich ELISAs

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Non-Mammalian-based, 1X

From $68.25

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