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Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Adult rat brain membrane immunoblot.Adult rat brain immunohistochemistry.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag- tagged REEP1, REEP2 or untagged Kv2.1 plasmid and probed with N345/51 (left) and K89/34 (right) TC supe.
Immunocytochemistry of mouse BV2 microglia cells treated with DMSO, DMSO+lipopolysaccharide, SMSO+SP600125 (a JNK inhibitor), showing specific staining of RELM alpha in green and nuclear staining in blue. Western Blot of transfected 293T cells showing specific immunolabeling of RELM alpha.
Western Blot of transfected HEK 293T cell lysate showing specific immunolabeling of secreted mouse RELM-beta from media.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Adult rat brain membrane immunoblot.

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