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Figure 1. Use of ELISA Blocking Buffer in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies ELISA Blocking Buffer

From $52.25

Figure 1. Use of ELISA Blocking Buffer - Non-Mammalian-based in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies ELISA Blocking Buffer - Non-Mammalian-based

From $54.25

ImmunoChemistry Technologies ELISA Plate Sealing Covers

$10

ImmunoChemistry Technologies ELISA Wash Buffer, 10X

From $44.75

Antibodies Incorporated End Point Titer Positive Control

$65

ImmunoChemistry Technologies Endogenous Peroxidase Block

From $58

ImmunoChemistry Technologies Eosin Y

From $58

Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Eosinophil Peroxidase Detection Kit

$475

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Equine Annexin V-Fluorescein Assay (Cat. 9141). Equine Annexin V-Fluorescene labeled only 17.4% of the control cells (UR + LR = 10.3% + 7.1%, left); staurosporine induced Equine Annexin V-Fluorescene labeling in 97.6% of cells (UR + LR = 27.8% + 69.8%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:68.

ImmunoChemistry Technologies Equine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Flow cytometry analysis of Jurkat cells treated with FLICA® 660-YVAD-FMK. After spiking FLICA® into the cell culture media, the cells were treated with either a negative control (left) or nigericin (20 μM, no.6698) to induce caspase-1 activity (right) for 24 hours. The negative control induced caspase-1 activity in 6.8% of cells (left); treatment with nigericin induced caspase-1 activity in 57.3% of cells (right). This is a ratio of 8:1. Data courtesy of Mrs. Tracy Murphy, ICT, 230-01.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit

$256.50

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

$256

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed on a Molecular Devices Gemini XPS 96-well fluorescence plate reader. The excitation spectrum (grey) was generated using an emission of 760 nm. The emission spectrum (red) was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis inducing agent (right), then stained with 660-VAD-FMK (cat. 9120) for 1 hour. Cells were washed twice and read on an Accuri C6 flow cytometer. Treatment with the negative control induced caspase activity in only 8.2% of cells (left), whereas treatment with staurosporine induced caspase activity in 96.5% of cells (right). This is a ratio of 12:1. Data courtesy of Mrs. Tracy Murphy, ICT, 213:48.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Poly (active) Caspase (VAD) Assay Kit

$256.50

ImmunoChemistry Technologies Fast Red Super ™

$113.75

ImmunoChemistry Technologies Fixative

$27.95

Figure 1. K562 cells were stained with CFSE analog. Lymphocytes were added at: effector (E)/ target (T) ratios of 25:1, 50:1, and 100:1. Following incubation, 7AAD (live/dead) stain was added and cells analyzed via flow cytometry. % Cytotoxicity can be determined by an increase of target cells (lower right quadrant) moving into upper right quadrant and calculated by (R1/(R1+R2))*100.

Figure 2. A linear increase indicates the assay is optimized and an E/T ratio will be optimal for the experiments.

ImmunoChemistry Technologies Flow Cytometry Cytotoxicity Assay

$310

Aves Labs Fluoresceinated Chicken Anti-Human IgG Secondary Antibody

$130

Aves Labs Fluoresceinated Chicken Anti-Mouse IgG Secondary Antibody

$130

Aves Labs Fluoresceinated Chicken Anti-Rabbit IgG Secondary Antibody

$130

Aves Labs Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody

$125

ImmunoChemistry Technologies Fluorescent ADP Detection Kit

From $393

ImmunoChemistry Technologies Fluorescent ATP Detection Kit

From $393

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.

Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.

ImmunoChemistry Technologies Fluorescent Cell Proliferation Assay

From $114

Antibodies Incorporated Fluorescent Goat anti-Human IgG for ANA kit

$75

Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).

Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.

ImmunoChemistry Technologies Fluorescent Hypochlorite Detection Kit

$506

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