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ImmunoChemistry Technologies BSA (IgG and Protease free)

From $68.25

Antibodies Incorporated Buffered Glycerol Mounting Media

$45

ImmunoChemistry Technologies Camptothecin

From $33.25

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained with Canine Annexin V-Fluorescein Assay (cat. 9139). Annexin V-Fluorescein labeled only 15.6% of the control cells (UR + LR = 9.8% + 5.8%, left); staurosporine induced Annexin V-Fluorescein labeling in 97.3% of cells (UR + LR = 11.5% + 85.8%, right). Staurosporine did not significantly increase the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 224:69-71.

ImmunoChemistry Technologies Canine Annexin V-Fluorescein Apoptosis Assay Kit

$455

ImmunoChemistry Technologies Cathepsin B

$338

ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test

From $173

Figure 1. Quantification of 4 populations in each sample

ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Total Cytotoxicity Test

From $373

Antibodies Incorporated Centromere Positive Control

$150

Figure 1. Staining of Healthy Jurkat Cells Healthy Jurkat cells were stained with CFSE or left unstained and then analyzed by flow cytometry using an Accuri C6 flow cytometer equipped with a FL1 99% attenuation filter. Unstained Jurkat cells (A), stained Jurkat cells (B), and the corresponding overlay (C) are shown.

Figure 2. Identification of CFSE-stained Cells. K562 target cells were stained green with CFSE and then subjected to effector cells. Green stained target cells are easily identifiable when analyzed by FL1 vs. SSC on a flow cytometer. Target cells stained green with CFSE move to the right along the X-axis (FL1) of the plot (right) compared with unstained effector cells. This plot becomes particularly important when gating on target cells that are the same size as effector cells.

ImmunoChemistry Technologies CFSE Green Fluorescent Stain - for cell labelling and proliferation studies

$120.75

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.

ImmunoChemistry Technologies Chicken Annexin V-Fluorescein Apoptosis Assay Kit

$455

Aves Labs Chicken Anti-Goat IgG Secondary Antibody

$115

Aves Labs Chicken Anti-Human IgG Secondary Antibody

$115

Aves Labs Chicken Anti-Rabbit IgG Secondary Antibody

$115

ImmunoChemistry Technologies Chlorination and Oxidation Detection Kit by Cell Technology

$682

ImmunoChemistry Technologies Citrate Buffer, 10X

From $58

Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.

Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut’s reagent prior to conjugation.

ImmunoChemistry Technologies Conjugation-Ready HRP Maleimide

$220

ImmunoChemistry Technologies Costar® 96-Well Black Polystyrene Plate

$8.05

ImmunoChemistry Technologies Costar® 96-Well EIA/RIA Stripwell Plate

$7.30

ImmunoChemistry Technologies DAPI Nuclear Stain

$120.50

Figure 2. Recovery of 8-OHdG from urine. Urine samples were spiked with 8-OHdG, diluted as described in the protocol, analyzed using the 8-OHdG ELISA Kit. The y-intercept corresponds to the amount of 8-OHdG in unspiked urine. Error bars represent standard deviations obtained from multiple dilutions of each sample.

ImmunoChemistry Technologies DNA Damage ELISA Kit

$657

Figure 1. Jurkat cells were stimulated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Apoptotic cells show a loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.

Figure 2. HeLa Cells activated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Cells becoming apoptotic show loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.

ImmunoChemistry Technologies Dual Caspase 1 and Mitochondrial Membrane Potential Assay Kit

From $189

ImmunoChemistry Technologies Dual Caspase 3/7 and Mitochondrial Membrane Potential Assay Kit

From $189

ImmunoChemistry Technologies Dual Poly Caspase and Mitochondrial Membrane Potential Assay Kit

From $189

ImmunoChemistry Technologies EDTA Buffer, 10X

From $58

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