Our Plexin D1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin D1 (C-terminal region) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).
Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
PLXND1
195/215/250
Plexin D1 synthetic peptide (coupled to carrier protein) corresponding to amino acids from the C-terminal region of human Plexin D1. This sequence is well conserved in rat and mouse Plexin D1, and has some homology to other Plexin family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
Unconjugated
The antibody detects 215 and 195 kDa bands corresponding to the apparent molecular mass of Plexin D1 variants in human umbilical vein endothelial cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Mazzotta, C., et al. 2015. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Research and Therapy, 221.
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