Monoclonal Antibodies

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Adult rat hippocampus immunohistochemistry.Immunoblot against crude rat brain membrane fractions (RBM) and extracts of COS cells transiently transfected with GFP-tagged rat RGS14 (rRGS14), Flag-tagged human RGS14 (hRGS14), GFP-tagged rat RGS12 (rRGS12) or untagged Kv2.1 plasmid probed with N133/35 (left) or N52A/42 (right) TC supe.
Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1, -2, -3, -4 and -4* plasmids and probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.Adult rat brain immunohistochemistry.
Whole brain immunohistochemistry (with antigen retrieval via Na citrate pretreatment).Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-MOG Antibody
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Adult rat hippocampus immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with V5-tagged AMIGO-1, AMIGO-2, AMIGO-3 or untagged Kv2.1 plasmid and probed with L86A/37 TC supe (left) plasmid and probed with L86A/37 TC supe (left).
Array tomography immunofluorescence of a single 70 nm LRWhite-embedded section from adult mouse cortex with L122/6 (green, left), rabbit Calretinin (red, right) and DAPI (blue).  Images courtesy of Kristina Micheva (Stanford)..Adult rat cortex immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with Myc-tagged SYN1, SYN3 or untagged Kv2.1 plasmid probed with L125/121 (left) or 9E10 (right) TC supe..
Adult rat cerebellum immunohistochemistry.Adult rat cortex immunohistochemistry.
Array tomography immunofluorescence of a Lowicryl-embedded 70 nm section from adult mouse cortex with L120/12 (green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford)..Adult rat brain membrane immunoblot.
Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex layer 2/3 with L113/130 (green), rabbit mAb PSD-95 (Cell Signaling #3450, red) and DAPI (blue), where L113/130 colocalizes with many PSD-95 puncta.  Image courtesy of Kristina Micheva (Stanford)..Adult rat hippocampus immunohistochemistry.
Immunoblot of crude membrane fractions from adult heart ventricle (V) and brain (B) from wild-type (WT) and Kvß1 knockout (Kvβ1) mice probed with K47/42 (Kvβ1.2, top) and K17/70 (Kvβ2, bottom). Data courtesy of Jeanne Nerbonne, Washington University SOM. Reproduced with permission from 2005 Aimond et al. Circ Res.Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc/Flag-tagged Kv1.7 or untagged Kv2.1 plasmid probed with N471/27 (left) or rabbit anti-Flag (right)..
Adult rat brainstem immunohistochemistry of motor trigeminal nucleus (A) and facial motor nucleus (B). Scale bar = 500 µm.
Immunoblot against extracts of COS cells transiently transfected with Myc/Flag-tagged TWIK-2 or untagged Kv2.1 plasmid probed with N469/28 (left) or rabbit anti-Flag (right)..
Immunofluorescence staining of brain sections from adult Kir3.3 wild-type (WT) and knockout (KO) mouse cortex with N455/15 (Kir3.3, red) and K89/34 (Kv2.1, blue) TC supe. Tissue courtesy of Candice Contet (The Scripps Research Institute)..Immunofluorescence staining of brain sections from adult Kir3.3 wild-type (WT) and knockout (KO) mouse cerebellum with N455/15 (Kir3.3, red) and K89/34 (Kv2.1, blue) TC supe. Tissue courtesy of Candice Contet (The Scripps Research Institute)..
Adult rat hippocampus immunohistochemistry.

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