Monoclonal Antibodies

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Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Frataxin and untagged Kv2.1 plasmids and probed with N191/7 (left), 19E10 (middle) and N52A/42 (right) TC supe.
Adult rat striatum immunohistochemistry.
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Cerebellar lysate immunoblot probed with N151/3 (3 mg/mL).Cultured rat hippocampal neuron surface (S, left) and total (T, right) protein immunofluorescence staining (green) with N151/3 (2 mg/mL).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/17 (left) and N159/5 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma-protocadherin-B2 and -B1 and untagged Kv2.1 plasmids and probed with N148/30 (left) and N86/6 (right) TC supe.
Immunolabeling of mouse hippocampus identifying GKAP (Anti-SAPAP, Cat no 75-156, green). Nuclear staining done with DAPI.  Image kindly provided by Huaye Zhang, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School. Adult RBM and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged SAPAP1, SAPAP4 or untagged Kv2.1 plasmids and probed with N127/31 TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblots versus crude adult rat brain membranes (RBM) and brain extracts from Thorase WT and KO mice and probed with N125/10 (left) and N52A/42 (right) TC supe. Samples courtesy of Dr. Jianmin Zhang, Dr. Ted Dawson and Dr. Valina Dawson, Johns Hopkins School of Medicine.
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N105/17 (left) or N52A/42 (right) TC supe.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and lethargic (lh/lh) mice (left) Lethargic mice courtesy of Dr. Jeff Noebels, Baylor College of Medicine.Adult rat brain membrane immunoblot.
Immunoblot against crude membranes from adult rat brain (RBM), wild-type (MBM-WT) and BKAlpha knockout (MBM- BKaKO) mouse brains probed with L6/23 (left), L6/60 (middle) or N52A/42 (right) TC supe. Reproduced from PLoS One (2010 Wersinger et al, PMID 21079807).Maximum intensity projections of confocal z stacks taken from the apical cochlear region of Eps8−/− (top) and Eps8−/− rescued (bottom) mice using antibodies against BK channels carrying IK,f (red) and the hair-cell marker Myo7a (white). Image from publication CC-BY-4.0. PMID: 36034774
Adult rat brain membrane immunoblot.
Adult rat optic nerve immunofluorescence staining L11A/41 (5 μg/ml)=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband.Adult rat brain membrane immunoblot.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat olfactory bulb immunohistochemistry.Adult rat cerebellum immunohistochemistry.
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and KChIP3 KO mouse brains probed with K66/38 (left) and K55/82 (right) TC supe. Mouse samples courtesy of Takeshi Sakurai and Joseph Buxbaum (Mount Sinai School of Medicine).
Immunofluorescence staining transiently Kv1.6-transfected COS cells.Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.6-transfected (COS+) and untransfected (COS-) COS cells.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.

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