Monoclonal Antibodies

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Immunoblot versus crude membrane preparations from adult rat brain (RBM) and Brevican wild-type (WT) and knockout (KO) mice and probed with N294A/6 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Matthew Rasband, Baylor College of Medicine.
Immunofluorescence staining of zebrafish sections at 5 days post- fertilization with N286/74 (green) and Synapsin 1/2 rabbit polyclonal (red). Left: optic tectum (TeO), inner plexiform layer (asterisk) and photoreceptor layer (arrow). Right: synapse-rich axonal projections in optic tectum. Image courtesy of Philip Washbourne (University of Oregon).Immunoblot versus homogenates from adult zebrafish muscle (Non- neuronal), brain (Neuronal) and purified brain postsynaptic densities (PSD) probed with N286/74 TC supe. Image courtesy of Philip Washbourne (University of Oregon).
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N270/47 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin plasmid and probed with N269/73 (left) and N86/8 (right) TC supe.
Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Immunoblot against adult rat brain membrane (RBM) or low- speed pellet (RBLSP) fractions denatured at 95oC for 5 min or 37oC for 15 min and probed with N253/32 (left) and K58/35 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with DsRed-tagged GABAARAlpha4, Flag-tagged GABAARAlpha6 or untagged Kv2.1 plasmid probed with N229A/32 (left), Mouse anti-Flag (middle) or N398A/34 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Lhx6.1 and untagged Kv2.1 plasmids and probed with N228A/16 (left) and 19E10 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Pex7 or untagged Kv2.1 plasmid and probed with N232/9 (left) and 19E10 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged FGF13B/FHF2B, FGF13A/FHF2A or untagged Kv2.1 plasmid and probed with N225A/10 (left) or N86/8 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/7 (left) or N86/8 (right) TC supe.
KC rabbit anti- Kv2.1 Brain samples courtesy of Albee Messing, UW- Madison.Immunofluorescence of GFAP WT and KO mouse hippocampus with N206B/9  Brain samples courtesy of Albee Messing, UW- Madison.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged INPP4b or untagged Kv2.1 plasmid and probed with N171/17 (left) and N86/8 (right) TC supe.Immunoblot of Inpp4b+/+ or Inpp4b−/−  mouse embryonic fibroblasts treated with vehicle or apilimod 10 nM for 48 h and assessed for protein levels of Inpp4b, LC3, and Beta actin as loading control. Image from publication CC-BY-4.0. PMID: 35760104
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.
Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).
Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).Transfected HEK293 cell immunofluorescence staining N15/39=green, DAPI=blue.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) or untagged Kv2.1 plasmid and probed with N129/15 (left) and K89/41 (right) TC supes.
Bacterial fusion protein immunoblot.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.2 or untagged Kv2.1 plasmids and probed with N124B/38 TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.Adult rat hippocampus immunohistochemistry.
Bacterially expressed GST fusion protein immunoblot.
NeuroMab Anti-GST Antibody
Sale price$319
Adult rat hippocampus immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with V5-tagged AMIGO-1, AMIGO-2, AMIGO-3 or untagged Kv2.1 plasmid and probed with L86/36 TC supe (left) and Mouse anti-V5 (right.

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