Monoclonal Antibodies

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Immunoblot versus RBM and lysates of WT and AMIGO-1 KO mouse brains probed with L86/33 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus lysates of whole rat brain (WBH) and of COS-1 cells transfected with human and rat MMP9 (hMMP9 and rMMP9, respectively) and Kv2.1 plasmids and probed with L51/82 (left), K89/34 (middle) and loading control (right) TC supe.
Cultured hippocampal neurons (9 DIV) double- Stained for MAP2 (red), L5/1 (green).Adult rat neocortex immunohistochemistry.
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
Adult whole rat brain homogenate immunoblot.Transfected cell immunoblot: extracts of COS-7 cells transiently transfected with SynCAM1, SynCAM2 or SynCAM3 plasmids and probed with L45/30 TC supe. Image courtesy of Dr. Philip Washbourne, Institute of Neuroscience, University of Oregon.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with BKBeta4 or Kv2.1 plasmids and probed with L18A/3 pure IgG at 10 mg/mL (left panel) or K89/41 TC supe (right panel).
Adult rat cortex hippocampus immunohistochemistry.Adult rat cortex immunohistochemistry.
Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue).  Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford)..Adult rat brain hippocampus immunohistochemistry.
adult rat hippocampus immunohistochemistryadult rat striatum immunohistochemistry
Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) or N52A/42 (right) TC supe.Adult rat whole brain immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot on adult rat brain membranes.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K91/36 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Transfected cell immunofluorescence: COS-1 cells expressing myc-tagged human Co-Rest. Red= K72/8, Green=anti-myc, Blue=DAPI.Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with human CoRest (left lane) or rat Kv2.1 (right lane) plasmids and probed with K72/8.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K40/17 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot
Adult rat brain membrane immunoblot
"Endogenous and transfected cell immunoblot: extracts of HEK-293 cells transfected with GFP-tagged LRRK2-WT, LRRK2-S910A or LRRK2-S935A plasmid; fibroblasts from embryonic WT or LRRK2 KO mice untreated or treated with LRRK2-IN-1 inhibitor (2011 Deng et al Nat Chem Biol); and human lymphoblasts untreated or treated with LRRK2-IN-1, probed with 1D8. Data courtesy of Nicolas Dzamko, Paul Davies and Dario Alessi (University of Dundee, Scotland, UK)."

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