Exciting New Immunohistochemistry (IHC) Products
for Enhanced Research!
ICT is excited to offer new products for IHC! Check out our Antigen Retrievers, Blocking Reagents, Buffers, Chromogens, Counter Stains and Mounting Media.
Preserve your stained specimens with confidence!
Mounting media is a solution used to preserve and protect stained tissue samples on microscope slides after the staining process. It is a critical component to help maintain the quality and longevity of the staining as well as to provide a clear and stable medium for microscopic examination. Choosing the right media is essential for protecting your samples and ensuring clear signals during viewing.
There are two categories of mounting media, organic and aqueous (or hydrophobic and hydrophilic, respectively).
Organic mounting media can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue (eg diaminobenzidine, DAB).
Aqueous mounting media is generally suitable for IHC because it is water-based, meaning it doesn't contain organic solvents that might dissolve the staining reactions. Aqueous mounting media suits most enzymatic label/chromogen combinations and fluorescent labels.
ICT's aqueous mounting media products include the popular Fluoroshield™.
Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears and is available in a variety of sizes and options:
- Fluoroshield™: Fluoroshield is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC. Coverslip is required. DIRECT SUBSTITUTE OF Biomeda’s Gel Mount
- Fluoroshield™ with DABCO: Fluoroshield with antifading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Alexa fluoro 488, Alexa fluoro 594, Cy dyes, tetramethyly rhodamine, and Redox. The fluorescence is retained during prolonged storage at 4oC in the dark. Coverslip is required.
- Fluoroshield™ with DAPI: Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. Coverslip is required.
- Fluoroshield™ with PPD: Fluoroshield with strong antifading agent, 1,4-phenylenediamine (PPD) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Alexa fluoro 488, Alexa fluoro 594, tetramethyly rhodamine, and Redox. The fluorescence is retained during prolonged storage at 4oC in the dark. This medium contains phenylenediamine and is not suitable for immunofluorescence of Cy dyes, GFP, mCherry, Phycoerythrin (R-PE), phyocyanin (PC), allophycocyanin (APC) and other fluorochromes proteins. Coverslip is required.
- Fluoroshield™ with DAPI and DABCO: Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
- Fluoroshield™ with DAPI and PG: Fluoroshield with DAPI and PG is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. May encounter problems with FROZEN BRAIN OR OTHER FROZEN TISSUES WITH LOTS OF FAT
- Fluoroshield™ with DAPI and PPD: Fluoroshield with DAPI and PPD is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Fluorescence is retained during prolonged storage at 4oC in the dark. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. This medium contains phenylenediamine and is not suitable for immunofluorescence of Cy dyes, GFP, mCherry, Phycoerythrin (R-PE), phyocyanin (PC), allophycocyanin (APC) and other fluorochromes proteins.
- Fluoroshield™ with PI: Fluoroshield mounting medium with PI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with PI which is a counter-stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. PI excites at 535nmand emits at 615nm, producing a red fluorescence. RNA is also stained with PI. Coverslip is required.
Additional IHC Reagents coming soon: Detergents, IHC Antibodies (monoclonal and polyclonal), IHC Kits, and Proteins/Protein Conjugates. Look for these to be available online shortly!