Ad 5 ARM (Adenovirus Reference Material) Wild-Type Adenovirus Particle
Our Ad 5 (Adenovirus Reference Material) wild-type adenovirus particle from ViraQuest is produced in-house. It has a wild-type viral backbone, and a titer of 1 to 1.3 e12 particles/mL. Contact us for custom adenovirus production services.
SKU: 92-VQ003
Product Details
Adenovirus Reference Material (ARM) is a standardized viral preparation used as a benchmark in studying adenovirus-based gene therapies and vaccines. ARM typically consists of a well-characterized adenovirus type (such as Ad5) and is used to define the particle unit and infectious unit for adenovirus-based gene vectors and to establish a reference point for comparative studies in vector development and potency assessments. The NIH Recombinant DNA Advisory Committee recommended the development of such a reference-testing agent in their report issued January 2002 (PMID: 11779411). The ARM was developed under the guidance of the Adenovirus Reference Material Working Group (ARMWG) and the U.S. FDA through the donation of services and supplies by a large number of laboratories and organizations from North America and Europe.
Viruses are purified over 2 rounds of CsCl gradients and then dialyzed and formulated in A195 buffer.
1 to 1.3 e12 particles/mL
Wildtype
n/a
HAdV-5
Store virus at -80 °C and avoid freeze-thaw cycles. Only make aliquots in the original buffer at the original concentration. Aliquots should be no less than 50 µL and stored in a screw cap tube.
Liquid
Viruses are purified over 2 rounds of CsCl gradients and then dialyzed and formulated in A195 buffer.
A195 buffer consisting of 10 mM Tris, 75 mM NaCl, 5% Sucrose (w/v), 0.020% PS-80 (w/v), 1 mM MgCl2, 0.1 mM EDTA, 0.5% EtOH (v/v), 10 mM Histidine, pH at 25°C = 7.4
United States
2 years from the date of receipt
Overnight shipping on dry ice
AY339865.1