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Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse  monoclonal anti-Girdin (lanes 1 & 2)  or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells
were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight® 594.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long, untagged Kirrel2, Kirrel1 or Kv2.1 plasmid and probed with N321C/49 (left) and N52A/42 (right) TC supe.

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