Anti-6xHis Antibody FL490 Conjugate (N144/14)

Name: Anti-6xHis Antibody FL490 Conjugate
Brand: NeuroMab
SKU: 75-169-FL490
Price: 460.0 USD
Availability: InStock

Our Anti-6xHis mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/14. It detects 6xHis, and is purified by Protein A chromatography. It is great for use in ICC.

Species Reactivity

Rat

Applications

ICC, IHC

Host Species

Mouse

Available Conjugates

Unconjugated

550

594

650

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Data Sheet

SKU: 75-169-FL490

Volume: 200 µL

Variant

Price:

$460.00

Quantity:

Product Details

Target

6xHis

Target Description

6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.

Format

Purified by Protein A chromatography

Concentration

0.5 mg/mL

Clonality

Monoclonal

Clone

N144/14

Isotype

IgG1

Applications

ICC, IHC

Host Species

Mouse

Gene Name

N/A

Molecular Weight

1 kDa

Antigen

Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.

Species Reactivity

Rat

Antibody Registry ID

AB_2939620

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Shipping

Shipped on ice packs

UniProt Details

UniProt (Human): N/A
UniProt (Immunogen Species): N/A

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Anti-6xHis Antibody FL490 Conjugate (N144/14)

SKU: 75-169-FL490

Product Details

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