Anti-AMIGO-1 Antibody (L86A/37)

Name: Anti-AMIGO-1 Antibody
Brand: NeuroMab
SKU: 75-329
Price: 319.0 USD
Availability: InStock

Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86A/37. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with V5-tagged AMIGO-1, AMIGO-2, AMIGO-3 or untagged Kv2.1 plasmid and probed with L86A/37 TC supe (left) plasmid and probed with L86A/37 TC supe (left).

Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and AMIGO-1 KO mouse brains probed with L86A/37 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-329

Product Details

Target

AMIGO-1

Target Description

Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L86A/37

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Amigo1 Ali2 Amigo Kiaa1163

Molecular Weight

60-80 kDa depending on maturity/glycosylation

Antigen

Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1 (accession number Q86WK6) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315802

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)

UniProt Details

UniProt (Human): Q86WK6
UniProt (Immunogen Species): Q80ZD8

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Human | Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32708917	1:500	Dulla, C.G., et al. 2020. Regulation of neurogenesis in mouse brain by HMGB1.. Cells, 1714.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
29403353	not listed	Bishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Frontiers in Molecular Science, 1.
24962901	not listed	Mandikian, D., et al. 2014. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors.. Journal of Comparative Neurology, 3555-3574.

Immunohistochemistry: Rat
PMID	Dilution	Publication
37758930	not listed	Mitchell, K.G., et al. 2023. High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Scientific Reports, 16200.

Western Blot: Human
PMID	Dilution	Publication
34137443	1:1000	Maverick, E.E., et al. 2021. Kv2 channel-AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking. Journal of Cell Science, jcs256339.

Western Blot: Mouse
PMID	Dilution	Publication
38590146	1:5 (supe)	Raja, R, et al. 2024. Insertion of a neomycin selection cassette in the Amigo1 locus alters gene expression in the olfactory epithelium leading to region-specific defects in olfactory receptor neuron development. Genesis, e23594.
32708917	1:1000	Dulla, C.G., et al. 2020. Regulation of neurogenesis in mouse brain by HMGB1.. Cells, 1714.

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Anti-AMIGO-1 Antibody (L86A/37)

SKU: 75-329

Product Details

Product Specific References for Applications and Species

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