Anti-Arp3 (C-terminal region) Antibody

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Our Arp3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Arp3 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).
Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).
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Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).
Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Chicken, Frog, Human, Mouse, Rat
ICC, IHC, WB
Rabbit

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Data Sheet

SKU: AP4581

Volume: 100 µL
Price:
$285.00
Ships: 1-2 business days

Product Details

Arp3 (C-terminal region)
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IHC, WB
Rabbit
ACTR3
47
Arp3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins.
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 1:100
ICC: 1:250
Unconjugated
The antibody detects a 47 kDa* doublet corresponding to the molecular mass of Arp3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
ACTR3, Arp3
P61158

Product Specific References for Applications and Species

GST pulldown assay: Mouse
PMID Dilution Publication
310634591:100Galvan, D, et al. 2019. Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice. Journal of clinical investigation, 2807-2823.
Immunohistochemistry: Human
PMID Dilution Publication
39472297not listedSun, Y, et al. 2024. ARP2/3 complex affects myofibroblast differentiation and migration in pancreatic ductal adenocarcinoma. International Journal of Cancer, .
Immunohistochemistry: Mouse
PMID Dilution Publication
33388318not listedZhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.
31063459not listedGalvan, D, et al. 2019. Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice. Journal of clinical investigation, 2807-2823.
Western Blot: Mouse
PMID Dilution Publication
33388318not listedZhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.

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