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ImmunoChemistry Technologies Immuno HRP-AEC, Anti-Chicken IgY (H+L)

From $293

ImmunoChemistry Technologies Immuno HRP-DAB, Anti-Chicken IgY (H+L)

From $293

ImmunoChemistry Technologies Immuno In Situ Mount™

From $88

ImmunoChemistry Technologies Immuno Mount™

From $77

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Donkey Serum

From $60

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Goat Serum

From $60

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Rabbit Serum

From $60

ImmunoChemistry Technologies Immuno Red Aqueous Counter Stain™

From $60

ImmunoChemistry Technologies ImmunoFluoroMount™ with PPD

From $83

ImmunoChemistry Technologies ImmunoHistoMount™

From $88

ImmunoChemistry Technologies ImmunoHistozyme

From $70

PhosphoSolutions iNOS Phospho-Regulation Antibody Kit

$459

PhosphoSolutions Integrin β1 (Human) Paired ELISA KIT

$459

PhosphoSolutions Integrin β4 Phospho-Regulation Antibody Kit

$459

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (middle), then stained using Intracellular GSH Assay (cat. 9137). Cells were read on a flow cytometer using an FL1 99% (2 log) attenuation filter. The median fluorescence intensity of negative control cells was 425,971 in FL1-A (left: Negative), and 289,169 in the induced cells (middle: Positive), a decrease of >30%. Overlay: green: Negative, red: Positive. Data courtesy of Ms. T. Hanson, ICT, 216:52, 051612.

ImmunoChemistry Technologies Intracellular GSH Assay Kit

$246

Figure 1. Jurkat cells were stained with the Intracellular Total ROS Green dye (cat. 9144), then treated with a negative control (left) or 100 µM tert-Butyl hydroperoxide (ROS-inducing agent) (middle). The median fluorescence intensity (MFI) of stained negative control cells was 11,875 in FL1-A (Negative) and 419,067 for ROS-induced cells (Positive). The data are also shown in overlay (black: Negative, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.

ImmunoChemistry Technologies Intracellular Total ROS Activity Assay

$352

PhosphoSolutions IκBα Phospho-Regulation Antibody Kit

$459

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine for 2 hours to induce apoptosis. Cells were then stained with JC-1 dye. Normal healthy cells (two cells, upper and lower left) concentrate JC-1 in mitochondria and fluoresce bright orange. Apoptotic cells (three cells, right), with mitochondria of various stages of permeability, exhibit a reduced orange fluorescence and increased green fluorescence, as JC-1 disperses through the cells. (Dr. Brian Lee, ICT).

Figure 2. Jurkat and HL-60 cells were exposed to DMSO (negative control) (dark orange) or 50 μM CCCP depolarizing agent (light orange) and then incubated with JC-1 for 20 minutes at 37°C, and washed. Aliquots were analyzed in triplicate in a fluorescence plate reader set at 488 nm excitation and 590 nm emission filter settings. Healthy cells exhibited a high level of orange fluorescence; the CCCP-depolarized cells had reduced orange fluorescence as the dye dispersed. (Ms. Tracy Hanson, ICT)

ImmunoChemistry Technologies JC-1 Fluorescent Mitochondrial Membrane Depolarization Assay

From $232.80

PhosphoSolutions JNK Phospho-Regulation Staining Kit

$459

PhosphoSolutions LIMK Phospho-Regulation Antibody Kit

$459

ImmunoChemistry Technologies Lysozyme Biotin, Conjugated

$246

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were exposed to 0.15 µM camptothecin (cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 µg/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.

ImmunoChemistry Technologies Magic Red® Fluorescent Caspase-3/7 Assay Kit

From $205.70

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin B Assay Kit

From $212.20

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $212.20

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