Anti-GABA-A-R-Gamma2L/S Antibody (N452/30)

Name: Anti-GABA-A-R-Gamma2L/S Antibody
Brand: NeuroMab
SKU: 75-483
Price: 319.0 USD
Availability: InStock

1 Citation

Our Anti-GABA-A-R-Gamma2L/S mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N452/30. It detects human, mouse, and rat GABA-A-R-Gamma2L/S, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot against extracts of COS cells transiently transfected with HA-tagged GABA-A-R-Gamma2L, GABA-A-R-Gamma2S or untagged Kv2.1 plasmid probed with N452/30 (left) or rabbit anti-HA (right)..

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-483

Product Details

Target

GABA-A-R-Gamma2L/S

Target Description

Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR γ2 subunit has two forms, designated “short” (γ2S) and “long” (γ2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N452/30

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GABRG2

Molecular Weight

45-55 kDa

Antigen

Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (accession number P18507-2) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2725814

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

GABA-A-R-Gamma2L Gamma-aminobutyric acid receptor subunit gamma-2) (GABRG2)

UniProt Details

UniProt (Human): P18507
UniProt (Immunogen Species): P18507-2

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
38623967	1:1000	Bundy, J, et al. 2024. Role of β3 subunit of the GABA type A receptor in triple negative breast cancer proliferation, migration, and cell cycle progression. Cell Cycle, 1-18.

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Anti-GABA-A-R-Gamma2L/S Antibody (N452/30)

SKU: 75-483

Product Details

Product Specific References for Applications and Species

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