Anti-GluN2A/NR2A Antibody (N327/95)

Name: Anti-GluN2A/NR2A Antibody
Brand: NeuroMab
SKU: 75-288
Price: 374.0 USD
Availability: InStock

Our Anti-GluN2A/NR2A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N327/95. It detects human, mouse, rabbit, and rat GluN2A/NR2A, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemstry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327/95 (left) and N86/38 (right) TC supe.

GST-pulldown experiments on adult rat hippocampus and cortex lysates using empty-GST or GST fused to the C-terminus of TSPAN5 (GST-Ct). Input: 2.5% of pulldown volume. Blots probed for GluA2/3, GluA1, Stargazin, and NMDAR subunit GluN2A (cat. 75-288, 1:1000). Image from publication CC-BY-4.0. PMID:36795458

Adult rat hippocampus immunohistochemstry.

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

SKU: 75-288

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

GluN2A/NR2A

Target Description

Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N327/95

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Grin2a

Molecular Weight

170 kDa

Antigen

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (accession number Q00959) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2315842

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GluN2B/NR2B

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A)

UniProt Details

UniProt (Human): Q12879
UniProt (Immunogen Species): Q00959

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Mouse | Rat
QMI: Mouse
Western Blot: Mouse | Rabbit | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
22922712	1:400	Ricciardi, S., et al. 2012. CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.. Nature Cell Biology, 911-923.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
39120267	1:50	Griffin, H, et al. 2024. MC4R Localizes at Excitatory Postsynaptic and Peri-Postsynaptic Sites of Hypothalamic Neurons in Primary Culture. Cells, 1235.
34871934	1:300	Gottesman, N., et al. 2021. ZnT1 is a neuronal Zn2+/Ca2+ exchanger. Cell Calcium, 102505.
33593425	1:1000	Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.
32147734	1:300	Koch, N., et al. 2020. Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms. Cerebral Cortex, 4306–4324.

Immunocytochemistry: Rat
PMID	Dilution	Publication
37099425	not listed	Falkovich, R., et al. 2023. A Synaptic Molecular Dependency Network in Knockdown of Autism- and Schizophrenia-Associated Genes Revealed by Multiplexed Imaging. Cell Reports, 112430.
35177616	1:250	Hruska, M., et al. 2022. Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific. Nature Communications, 920.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33593425	1:1000	Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.

Immunohistochemistry: Rat
PMID	Dilution	Publication
25822337	5 µg/mL	Balsara, R., et al. 2015. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke.. PLoS One, e0122840.

Quantitative Multiplex Co-Immunoprecipitation: Mouse
PMID	Dilution	Publication
34852231	not listed	Lautz, J.D., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Reports, 110076.

Western Blot: Mouse
PMID	Dilution	Publication
36681359	1:500	Woodburn, S.C., et al. 2023. Depletion of Microglial BDNF Increases Susceptibility to the Behavioral and Synaptic Effects of Chronic Unpredictable Stress. Brain, Behavior, and Immunity, 127-138.
33593425	1:1000	Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.
33402706	not listed	Mossa, A., et al. 2021. Developmental impaired Akt signaling in the Shank1 and Shank3 double knock-out mice. Molecular Psychiatry, 1928-1944.
33158444	1:500	Espinoza, S., et al. 2020. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biology, 164.
29069439	1:100	Shahin, S., et al. 2018. 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling. Toxicological Sciences, 349-374.
27098698	1:10 (supe)	D'Orsi, B., et al. 2016. Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury. The Journal of Neuroscience, 4564-4578.
26714288	not listed	Moraga-Amaro, R., et al. 2016. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.. Neuropharmacology, 222-235.
25632145	1:10 (supe)	D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. The Journal of Neuroscience, 1706-1722.
25069643	not listed	Bhattacharyya, M., et al. 2014. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832.
24360280	not listed	Parkhurst, C.N., et al. 2013. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor.. Cell, 1596-609.
23871843	not listed	Bortolato, M., et al. 2013. Early postnatal inhibition of serotonin synthesis results in long-term reductions of perseverative behaviors, but not aggression, in MAO A-deficient mice. Neuropharmacology, 223-232.

Western Blot: Rabbit
PMID	Dilution	Publication
25069643	not listed	Bhattacharyya, M., et al. 2014. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832.

Western Blot: Rat
PMID	Dilution	Publication
36795458	1:1000	Moretto, E., et al. 2023. The Tetraspanin TSPAN5 Regulates AMPAR Exocytosis by Interacting with the AP4 Complex. eLife, e76425.
35862168	1:500	Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360.
34375626	not listed	Moraga-Amaro, R., et al. 2021. Prenatal fluoxetine impairs non-hippocampal but not hippocampal memory in adult male rat offspring. Neuropharmacology, 108751.
29487522	1:500	Uttl, L., et al. 2018. Chronic MK-801 Application in Adolescence and Early Adulthood: A Spatial Working Memory Deficit in Adult Long-Evans Rats But No Changes in the Hippocampal NMDA Receptor Subunits. Frontiers in Pharmacology, .
27458019	1:1000	Xie, J.D., et al. 2016. Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain*. Molecular Bases of Disease, 19364-19373.
24103031	1:800	Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. The FEBS Journal, 6114-27.

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Anti-GluN2A/NR2A Antibody (N327/95)

SKU: 75-288

Product Details

Product Specific References for Applications and Species

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