Anti-mCherry Antibody

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Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mCherry, and is affinity purified. It is great for use in ICC, WB.

Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
IMCD3 cells transfected with Arl13b-mCherry​ and stained with Anti-mCherry Antibody (cat. MCHERRY, 1:300) and visualized​ with A594 anti-chicken secondary antibody. ​ As expected, cytoplasmic and ciliary signals are observed in transfected cells.​ Image kindly provided by Svetlana Makova, Yale University.
Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.
Western blotting of recombinant fluorescent proteins with AvesLabs Chicken anti- mCherry antibody. The indicated (nanogram) amounts of each purified recombinant fluorescent protein was loaded on a gel and analyzed by Western blotting. AvesLabs Chicken anti-mCherry antibody recognizes ng amounts of mCherry protein and the related dsRED protein but does not show any reactivity with GFP.
Cross sections of the mylz2:EGFP and smyhc1:LY-Tomato double transgenic fish demarcating fast-twitch myofibers of the profound muscle (endogenous EGFP, cytosolic localization, green) and superficial layers (anti-Tomato/Cherry immunostaining (cat. MCHERRY), plasma membrane localization, red) of the slow muscle compartment, which is immunolabeled with Tnnt2 antibody (blue). All nuclei are stained with DAPI (white).
Immunostaining of zebrafish kidney tissue fixed in 4% paraformaldehyde (PFA) and paraffin embedded (FFPE) showing specific detection of mCherry (cat. MCHERRY, 1:1000; red). Nuclei stained with DAPI (blue). Image kindly provided by J. Ashley Ezzell, UNC Histology Research Core.
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Nkx2.1 (red) marks the epithelium and nuclei are labeled with DAPI (blue). Image kindly provided by Erica Yao, University of California San Francisco).
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Image kindly provided by Erica Yao, University of California San Francisco).
IF of a section of mouse brain NAc labeled with Anti-mCherry (1:1000, red), colabeled with eGFP, green, and DAPI staining of nuclear DNA. The Anti-mCherry specifically targeted the cpmApple-based membrane-localized red dopamine sensor co-expressed with eGFP in NAc and identified both cellular and subcellular distribution of the sensor. 20X/0.8 Air & 40X/1.1 Water Confocal, scale bar 100 um; 20 um. Donkey anti-chicken AlexaFluor647. Image from by Zikai Wang, Biomedical Engineering, USC.
Co-immunoprecipitation of truncated SCRIB with AnkG270. The experiment was performed once. IP immunoprecipitation, IB immunoblotting, Ck-GFP chicken anti-GFP, Ck-mCherry chicken anti-mCherry (cat. MCHERRY, 1:1000), Rb-GFP rabbit anti-GFP. Image from publication CC-BY-4.0. PMID:38081810

ICC, IHC, WB
Chicken

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Data Sheet
Trial Size Trial Size

SKU: MCHERRY-0100

Volume: 500 µL
Price:
$470.00
Ships: 1-2 business days

Product Details

mCherry
mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.
Affinity purified
0.2 mg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
MCHERRY
27 KDa for native monomer
Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli
AB_2910557
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:500-1:1000
ICC: 1:100-1:500
Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
Ice packs
X5DSL3

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
359413691:1000Sahu, S.K., et al. 2022. A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development. Nature Cell Biology, 1265-1277.
Immunohistochemistry: mouse
PMID Dilution Publication
393054851:500Ubina, T., et al. 2024. Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice. STAR Protocols, 103322.
Immunohistochemistry: Zebrafish
PMID Dilution Publication
383786931:200Oudhoff, H, et al. 2024. Skeletal muscle regeneration after extensive cryoinjury of caudal myomeres in adult zebrafish. NPJ Regenerative medicine, 8.
364952921:200Shin, K, et al. 2024. leptin b and its regeneration enhancer illustrate the regenerative features of zebrafish hearts. Developmental Dynamics, 91-106.
Western Blot: Rat
PMID Dilution Publication
380818101:1000Zhang, W., et al. 2023. Immunoproximity biotinylation reveals the axon initial segment proteome. Nature Communications, 8201.

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