Anti-P-Zero Myelin Protein Antibody

Name: Anti-P-Zero Myelin Protein Antibody
Brand: Aves Labs
SKU: PZO
Price: 455.0 USD
Availability: InStock

78 Citations

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_032649) and human (NP_000521) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

In the image (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.

Immunofluorescence of a section of injured (right, crushed) and normal (left) mouse sciatic nerve labeled with Anti-P-Zero Myelin Protein (1:1000, red), colabeled with Anti-Neurofilament NF-L (green), and DAPI labeling nuclei. Scale bar, 50 μm; Magnification, 20×. Image kindly provided by Prem Kumar, Department of Orthopedics and Rehabilitation, Penn State University College of Medicine, John C. Elfar lab.

Remyelinated lesion of index case stained for PLP (green) and Pzero (Cat No. PZO, red), showing that the myelin is distinctly from oligodendrocyte or Schwann cell origin, respectively. Image courtesy of Bryan Bollman, Washington University in St. Louis.

P-zero (brown staining) in all of the myelinating Schwann cells of a tissue section of an adult sciatic nerve.

Characterization of mouse vagus nerve derived Schwann cells (VNSCs). The identity and purity of SCs were confirmed using IF staining of S100 (pink), p75NTR (green), and Mpz (Cat. PZO, 1:1000, red) under a fluorescent microscope (ZEISS Apotome 2). The purity of the cultured SCs (99%) was analyzed by double positive staining of DAPI with S100/p75NTR/Mpz markers. Each image represents 3 images from 3 independent experiments. Scale bar: 50 μm, n = 3. Image from publication CC-BY-4.0. PMID: 37127673

Representative immunofluorescence images of mouse sciatic nerve teased fibers at postnatal day 30. Fibers were stained for P0 (cat. PZO; green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884

Representative immunofluorescence images of mouse sciatic nerve cross sections at 2 months of age. Cross sections were stained for P0 (cat. PZO; green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884

Western blot analysis of P0 (cat. PZO) in sciatic nerve lysate from wild-type (WT), MpzT124M/+ (TM/+), and MpzT124M/T124M (TM/TM) mice treated with (cut) or without (uncut) EndoH (A) and PNGase F (B). Image from publication CC-BY-4.0. PMID: 36350884

In mature myelinating cultures, SCs CASPR (cat.75-001, 1:500; white) can be detected in the characteristic staining pattern marking paranodes. Scale bar 5 μm. Paranodal CASPR co-localised on NFL-labelled axons (magenta). Scale bar: 5 μm. When colabelling with MPZ (cat. PZO, 1:500; green), CASPR is localised to paranodal loops adjacent to a node of Ranvier. Scale bar: 10 μm. Image from publication CC-BY-4.0. PMID:37642648

Confocal image of MPZ (cat. PZO, 1:500; green)-labelled myelinating mouse Schwann cells (SCs). Image from publication CC-BY-4.0. PMID:37642648

Western blot analysis of PMP2 and MPZ (cat. PZO, 1:5000) in control, Bace1−/−, NRG1t3OE, Bace1−/−; NRG1t3OE, NRG1t3ΔC (Constitutively active NRG1 type III) and Bace1−/−; NRG1t3ΔC at 60 days of age (C,D). Tubulin (TUB) was used for the endogenous control for the Western blots. Image from publication CC-BY-4.0. PMID:38311982

In the image (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

Trial Size

SKU: PZO

Volume: 1000 µL

Variant

Price:

$455.00

Quantity:

Product Details

Target

Myelin Protein Zero (Pz0)

Target Description

Human Myelin Protein Zero (Pz0) is a 28,400 dalton protein (258 amino acids) found in myelin of the PNS, but not the CNS. Expressed in myelinating Schwann cells, Pz0 serves as a homophilic adhesion molecule, allowing adjacent laminae of the myelin sheath to undergo compaction. Pz0 is also a marker of a subpopulation of migrating neural crest cells. Mutations of the MPZ gene product in humans is responsible for Charcot-Marie-Tooth type 1B neuropathies (HMSN1B).

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MPZ

Molecular Weight

27 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313561

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Myelin protein P0 (Myelin peripheral protein) (MPP) (Myelin protein zero)

UniProt Details

UniProt (Immunogen Species): O95297

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Human | Mouse | Rat | Swine
Immunoprecipitation: Mouse
Western Blot: Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
28512649	1:500	Stratton, J.A., et al. 2017. Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve. eNeuro, .

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37642648	1:500	Mutschler, C, et al. 2023. Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures. Journal of Cell Science, .
37127673	1:1000	Govindappa, P.K., et al. 2023. A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility. BMC Biotechnology, 12.
35296651	1:1000	Govindappa, P.K., et al. 2022. Erythropoietin promotes M2 macrophage phagocytosis of Schwann cells in peripheral nerve injury. Cell death & disease, 245.
34288120	1:500	Booth, D.G., et al. 2021. Characterizing the molecular etiology of arthrogryposis multiplex congenita in patients with LGI4 mutations. Glia, 2605-2617.
29976926	1:500	Volpi, V.G., et al. 2018. Zika Virus Replication in Dorsal Root Ganglia Explants from Interferon Receptor1 Knockout Mice Causes Myelin Degeneration. Scientific Reports, 10166.
29778523	1:250	Park, J., et al. 2018. Local Immunomodulation with Anti-inflammatory Cytokine-Encoding Lentivirus Enhances Functional Recovery after Spinal Cord . Molecular Therapy, 1756-1770.
16775154	1:500	McKenzie, I.A., et al. 2006. Skin-derived precursors generate myelinating Schwann cells for the injured and dysmyelinated nervous system. Journal of Neuroscience, 6651-6660.

Immunocytochemistry: Rat
PMID	Dilution	Publication
36222946	1:1000	Suzuki, T., et al. 2022. Molecular and Regenerative Characterization of Repair and Non-repair Schwann Cells. Cellular and Molecular Neurobiology, .
24391468	1:500	Lundgaard, I., et al. 2013. Neuregulin and BDNF induce a switch to NMDA receptor-dependent myelination by oligodendrocytes. PLoS Biology, e1001743.
20696161	not listed	Hossain, S., et al. 2010. Regulation of peripheral myelination by Src-like kinases. Experimental Neurology, 47-57.

Immunohistochemistry: Human
PMID	Dilution	Publication
28512649	1:500	Stratton, J.A., et al. 2017. Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve. eNeuro, .

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37768019	1:250	Ross, B.C., et al. 2023. Building‐Block Size Mediates Microporous Annealed Particle Hydrogel Tube Microenvironment Following Spinal Cord Injury. Advanced Healthcare Materials, e2302498.
35900443	1:500	Lee, J.I., et al. 2023. Functional recovery and muscle atrophy in pre-clinical models of peripheral nerve transection and gap-grafting in mice: effects of 4-aminopyridine. Neural Regenerative Research, 439-444.
36479906	not listed	Procacci, N.M., et al. 2022. Kir4.1 is Specifically Expressed and Active in Non-myelinating Schwann Cells. Glia, .
36350884	1:300	Shackleford, G., et al. 2022. A New Mouse Model Of Charcot-Marie-Tooth 2J Neuropathy Replicates Human Axonopathy And Suggest Alteration In Axo-Glia Communication. PLoS Genetics, e1010477.
36350884	1:300	Shackleford, G., et al. 2022. A New Mouse Model Of Charcot-Marie-Tooth 2J Neuropathy Replicates Human Axonopathy And Suggest Alteration In Axo-Glia Communication. PLoS Genetics, e1010477.
36307805	not listed	Manto, K.M., et al. 2022. Erythropoietin-PLGA-PEG as a Local Treatment to Promote Functional Recovery and Neurovascular Regeneration After Peripheral Nerve Injury. Journal of Nanobiotechnology, 461.
34240706	1:200	Malavasi, E.L., et al. 2021. Dynamic early clusters of nodal proteins contribute to node of Ranvier assembly during myelination of peripheral neurons. eLife, e68089.
33728392	1:200	Ehsanipour, A., et al. 2021. Injectable, macroporous scaffolds for delivery of therapeutic genes to the injured spinal cord. APL Bioengineering, 16104.
33538762	1:1000	Wang, M., et al. 2021. Completion of neuronal remodeling prompts myelination along developing motor axon branches. Cellular Neurobiology, .
33508430	1:250	Heller, G.J., et al. 2021. Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease. Molecular Therapy, 1883-1902.
33499508	1:500	Lee, J.I., et al. 2021. Purposeful Misalignment of Severed Nerve Stumps in a Standardized Transection Model Reveals Persistent Functional Deficit With Aberrant Neurofilament Distribution. Military Medicine, 696-703.
33303798	1:500	Lee, J.I., et al. 2020. A novel nerve transection and repair method in mice: histomorphometric analysis of nerves, blood vessels, and muscles with functional recovery. Scientific Reports, 21637.
32807950	1:1000	Babetto, E., et al. 2020. A glycolytic shift in Schwann cells supports injured axons. Nature Neuroscience, 1215-1228.
32672815	1:500	Wüst, H.M., et al. 2020. Egr2-guided histone H2B monoubiquitination is required for peripheral nervous system myelination. Nucleic Acids Research, 8959-8976.
32641402	1:800	Reed, C.B., et al. 2020. Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury. Journal of Neuroscience, 6165-6176.
32375064	not listed	Weinstock, N.I., et al. 2020. Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction. Neuron, 65-81.
32209430	not listed	Eshed-Eisenbach, Y., et al. 2020. Precise Spatiotemporal Control of Nodal Na+ Channel Clustering by Bone Morphogenetic Protein1/Tolloid-like Proteinases. Neuron, 806-815.
32143001	1:1000	Govindappa, P.K., et al. 2020. An effective erythropoietin dose regimen protects against severe nerve injury-induced pathophysiological changes with improved neural gene expression and enhances functional recovery. International Immunopharmacology, 106330.
32000627	not listed	Smith, D.R., et al. 2020. Polycistronic Delivery of IL-10 and NT-3 Promotes Oligodendrocyte Myelination and Functional Recovery in a Mouse Spinal Cord Injury Model. Tissue Engineering, 672-682.
33423486	not listed	Smith, D.R., et al. 2019. PLG bridge implantation in chronic SCI promotes axonal elongation and myelination. ACS Biomaterials Science & Engineering, 6679-6690.
31285339	1:250	Park, J., et al. 2019. Intravascular innate immune cells reprogrammed via intravenous nanoparticles to promote functional recovery after spinal cord injury. PNAS: USA, 14947-14954.
30901424	not listed	Noble, M., et al. 2019. 4-Aminopyridine as a Single Agent Diagnostic and Treatment for Severe Nerve Crush Injury. Military Medicine, 379-385.
30610918	1:250	Dumont, C.M., et al. 2018. Aligned hydrogel tubes guide regeneration following spinal cord injury. Acta Biomaterialia, 312-322.
30535360	1:1000	Belin, S., et al. 2018. Neuregulin 1 type III improves peripheral nerve myelination in a mouse model of Congenital Hypomyelinating Neuropathy. Human Molecular Genetics, 1260-1273.
30229864	not listed	Smith, D.R., et al. 2018. Combinatorial lentiviral gene delivery of pro‐oligodendrogenic factors to improve myelination of regenerating axons after spinal cord injury. Biotechnology and Bioengineering, 155-167.
30184491	1:200	Stratton, J.A., et al. 2018. Macrophages Regulate Schwann Cell Maturation after Nerve Injury. Cell Reports, 2561-2572.
29361054	1:2000	Truch, K., et al. 2018. Analysis of the human SOX10 mutation Q377X in mice and its implications for genotype-phenotype correlation in SOX10-related human disease.. Human Molecular Genetics, 1078-1092.
28760862	1:100	Assinck, P., et al. 2017. Myelinogenic Plasticity of Oligodendrocyte Precursor Cells following Spinal Cord Contusion Injury. The Journal of Neuroscience, 8635-8654.
28168703	1:1000	Geary, M.B., et al. 2016. Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle & Nerve, 143-151.
28139683	1:500	Brügger, V., et al. 2016. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nature Communications, 14272.
27593486	1:1000	Sundem, L., et al. 2016. Erythropoietin Enhanced Recovery After Traumatic Nerve Injury: Myelination and Localized Effects. The Journal of Hand Surgery, 999-1010.
24719088	not listed	Amor, V., et al. 2014. Long-term maintenance of Na+ channels at nodes of Ranvier depends on glial contact mediated by gliomedin and NrCAM. Journal of Neuroscience, 5089-5098.
23791981	1:500	Thomas, A.M., et al. 2013. Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury. Journal of Controlled Release, 421-429.
22764250	1:2000	Sherman, D.L., et al. 2012. Drp2 and periaxin form Cajal bands with dystroglycan but have distinct roles in Schwann cell growth. Journal of Neuroscience, 9419-9428.
22337502	1:100	Verdier, V., et al. 2012. Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways. Glia, 751-760.
22302821	1:200	Sherman, D.L., et al. 2012. Arrest of myelination and reduced axon growth when Schwann cells lack mTOR. Journal of Neuroscience, 1817-1825.
21363884	1:300	Fratta, P., et al. 2011. P0S63del impedes the arrival of wild-type P0 glycoprotein to myelin in CMT1B mice. Human Molecular Genetics, 2081-2090.
18373484	1:100	Plemel, J.R., et al. 2008. A graded forceps crush spinal cord injury model in mice. Journal of Neurotrauma, 350-370.

Immunohistochemistry: Rat
PMID	Dilution	Publication
35434551	not listed	Park, H., et al. 2022. ACTL6a coordinates axonal caliber recognition and myelination in the peripheral nerve.. iScience, 104132.
34519641	1:300	Nunes, G.D.F., et al. 2021. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination. Elife, e66278.
32559459	1:100	Assinck, P., et al. 2020. Transplantation of Skin Precursor-Derived Schwann Cells Yields Better Locomotor Outcomes and Reduces Bladder Pathology in Rats with Chronic Spinal Cord Injury. Stem Cell Reports, 140-155.
30511019	1:100	Estrada, V., et al. 2018. Low-pressure micro-mechanical re-adaptation device sustainably and effectively improves locomotor recovery from complete spinal cord injury. Nature Communications Biology, 205.
30184491	1:200	Stratton, J.A., et al. 2018. Macrophages Regulate Schwann Cell Maturation after Nerve Injury. Cell Reports, 2561-2572.
27889927	1:500	Wegener, A., et al. 2016. Sp2 is the only glutamine-rich Specificity protein with minor impact on development and differentiation in myelinating glia. Journal of Neurochemistry, 245-256.
26121489	1:200	Stratton, J.A., et al. 2015. The immunomodulatory properties of adult skin-derived precursor Schwann cells: implications for peripheral nerve injury therapy. European Journal of Neuroscience, 365-375.
27343803	1:1000	Sapio, M.R., et al. 2009. Transcriptomic analyses of genes and tissues in inherited sensory neuropathies. Experimental Neurology, 375-395.

Immunohistochemistry: Swine
PMID	Dilution	Publication
33862596	1:1000	Hellman, A., et al. 2021. Development of a common peroneal nerve injury model in domestic swine for the study of translational neuropathic pain treatments. Journal of Neurosurgery, 1-8.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
29076578	not listed	VerPlank, J.J.S., et al. 2018. Impairment of protein degradation and proteasome function in hereditary neuropathies. Glia, 379-395.

Western Blot: Mouse
PMID	Dilution	Publication
38311982	1:5000	Hong, J, et al. 2024. PMP2 regulates myelin thickening and ATP production during remyelination. Glia, 885-898.
36350884	1:5000	Shackleford, G., et al. 2022. A New Mouse Model Of Charcot-Marie-Tooth 2J Neuropathy Replicates Human Axonopathy And Suggest Alteration In Axo-Glia Communication. PLoS Genetics, e1010477.
33336855	1:5000	Jeanette, H., et al. 2020. YAP and TAZ regulate Schwann cell proliferation and differentiation during peripheral nerve regeneration. Glia, 1061-1074.
32641402	1:1000	Reed, C.B., et al. 2020. Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury. Journal of Neuroscience, 6165-6176.
31453649	1:4000	Jha, M.K., et al. 2020. Monocarboxylate transporter 1 in Schwann cells contributes to maintenance of sensory nerve myelination during aging. Glia, 161-177.
30901424	not listed	Noble, M., et al. 2019. 4-Aminopyridine as a Single Agent Diagnostic and Treatment for Severe Nerve Crush Injury. Military Medicine, 379-385.
30535360	1:5000	Belin, S., et al. 2018. Neuregulin 1 type III improves peripheral nerve myelination in a mouse model of Congenital Hypomyelinating Neuropathy. Human Molecular Genetics, 1260-1273.
29076578	not listed	VerPlank, J.J.S., et al. 2018. Impairment of protein degradation and proteasome function in hereditary neuropathies. Glia, 379-395.
29109239	1:2000	Fazal, S.V., et al. 2017. Graded Elevation of c-Jun in Schwann Cells In Vivo: Gene Dosage Determines Effects on Development, Remyelination, Tumorigenesis, and Hypomyelination. Journal of Neuroscience, 12297-12313.
28484008	not listed	Beirowski,B., et al. 2017. mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells. PNAS: USA, E4261-E4270.
28139683	1:1000	Brügger, V., et al. 2016. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nature Communications, 14272.
27861125	not listed	Tseng, K.C., et al. 2016. 4‐Aminopyridine promotes functional recovery and remyelination in acute peripheral nerve injury. EMBO Molecular Medicine, 1409-1420.
22302821	1:2000	Sherman, D.L., et al. 2012. Arrest of myelination and reduced axon growth when Schwann cells lack mTOR. Journal of Neuroscience, 1817-1825.
21363884	not listed	Fratta, P., et al. 2011. P0S63del impedes the arrival of wild-type P0 glycoprotein to myelin in CMT1B mice. Human Molecular Genetics, 2081-2090.

Western Blot: Rat
PMID	Dilution	Publication
25762662	not listed	Domenech-Estevez, E., et al. 2015. Distribution of Monocarboxylate Transporters in the Peripheral Nervous System Suggests Putative Roles in Lactate Shuttling and Myelination. The Journal of Neuroscience, 4151-4156.
20696161	not listed	Hossain, S., et al. 2010. Regulation of peripheral myelination by Src-like kinases. Experimental Neurology, 47-57.

Additional Publications: Unspecified
PMID	Publication
31479164	Forese, M.G., et al. 2020. Prostaglandin D2 synthase modulates macrophage activity and accumulation in injured peripheral nerves. Glia, 95-110.

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Anti-P-Zero Myelin Protein Antibody

SKU: PZO

Product Details

Product Specific References for Applications and Species

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