ICT’s Fixative is a formaldehyde solution designed to cross-link intracellular components. It will not interfere with the fluorescence labels used on our fluorescent detection probes like FLICA, Magic Red, and FLISP. Fixative is useful to preserve a cellular staining configuration for 24 hours before cellular analysis. Cells should be stained prior to fixation. This product is included in many of our cell viability assay kits, and can be utilized in a variety of cellular analysis workflows.
Warning: This product is toxic if inhaled, in contact with skin, or if swallowed. Gloves, protective clothing, and eyewear are strongly recommended. When disposing, flush sink with copious amounts of water.
2-8°C
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4.5 + 1.0
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If fluorescence-stained cells cannot be evaluated immediately after labeling with the fluorescence probes, add Fixative to the cells at a ratio of 1:10. For example, add 100 µL Fixative to 900 µL cells and incubate for at least 15 minutes at RT in the dark. Never add Fixative until the staining and final wash steps have been completed. Fixed cells may be stored on ice or at 4°C up to 24 hours.