Immuno Red Aqueous Counter Stain™
This Red aqueous counter Stain is uniquely formulated for staining of protein when NBT/BCIP is used in Immunohistochemistry or in Situ hybridization.
This Red aqueous counter Stain is uniquely formulated for staining of protein when NBT/BCIP is used in Immunohistochemistry or in Situ hybridization. NBT/BCIP gives purple blue color; the chromogen is soluble in organic solvents and cannot be mounted with organic mounting medium.
Ready to use counterstain
Room Temperature
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
In Situ hybridization, Immunohistochemistry, Immunocytochemistry.
- After chromogen staining, wash slide 2-3X with distilled or deiononized water.
- Apply Red aqueous counter stain for 3-5 minutes at RT.
- Rinse slide to be mounted with Distilled or DEIONIZED water, touch the edges of slide on a paper towel to remove excess water and place the slides on a flat surface.
- Apply 3-4 drops of Immuno In Situ mount mounting medium(AR-6507) directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Wait for 5 minutes. Excess mounting medium can be removed by touching the edges of slide against a paper towel.
- Let stand at room temperature for about 1 to 2 hours, or the slides can be heated at 37º- 40oC for 40-60 minutes. The slides are ready for visualization under a microscope, when 10X or 20X lenses are used.