ELISA Wash Buffer, 10X
ELISA Wash Buffer effectively rinses ELISA plates between reagent addition steps to remove signal-altering debris yet preserve assay components to reduce background and increase signal. Suitable for antibody-sandwich ELISAs and antigen-down ELISAs.
An optimal formulation of salts and detergents to wash ELISA plates.
ELISA Wash Buffer, 10X is used to remove unbound components from microtiter plates after the coating process and between reagent addition steps of an ELISA. ICT’s ELISA Wash Buffer is an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound assay components and interfering substances can be washed away without suppressing antigen-antibody binding interactions, thereby reducing non-specific background noise and increasing the specific signal.
ICT’s ELISA Wash Buffer, 10X is a universal ELISA wash buffer; it may be used with antibody-sandwich ELISAs and antigen-down ELISAs. It contains a non-azide, non-mercury preservative that will not interfere with the assay binding interactions, while still providing excellent long-term, 1X storage stability at room temperature. As it is supplied concentrated at 10X, crystalline precipitates may form in the bottle, especially when refrigerated. If this occurs, gently warm or mix the buffer until all crystals have dissolved.
- Dilute ELISA Wash Buffer, 10X by adding 1 part ELISA Wash Buffer to 9 parts deionized water and mix for 15 minutes. ELISA Wash Buffer, 10X is supplied concentrated, so crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil.
- Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 µL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system.
- Aspirate or dump out the buffer and repeat for a total of 2-4 washes.
- After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid. If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.
Product Specific References
| PMID | Publication |
| 37119513 | Furuta, R.A., et al. 2023. Development of a recombinant hepatitis B immunoglobulin derived from B cells collected from healthy individuals administered with hepatitis B virus vaccines: A feasibility study. Transfusion, 1204-1214. |