HRP Conjugate Stock Stabilizer, 5X
This reagent is best for users who need an all-purpose HRP conjugate diluent and stabilizer. It can serve as a base to add specialized components to address non-specific binding. It is an excellent concentrated buffer to store HRP conjugates.
Stabilizes antibody-HRP conjugates while minimizing non-specific signal.
HRP Conjugate Stock Stabilizer, 5X is provided as a 5X concentrate. When diluted to its 1X working concentration, it can be used to create concentrated stock conjugates for long-term storage, to reconstitute lyophilized HRP conjugates, and dilute protein-peroxidase conjugates to their useful working titer in ELISAs and other immunology-based techniques. At 1X, HRP Conjugate Stock Stabilizer maintains the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of the HRP-IgG conjugate complex. This proprietary formulation helps preserve the native three-dimensional conjugate structure during storage while concurrently enhancing the steric interaction or binding relationship between the IgG component of the HRP- IgG conjugate and the target antigen or antibody during the assay.
By maintaining conjugate component activity and preventing contamination issues through the inclusion of an anti-microbial agent, HRP Conjugate Stock Stabilizer extends the functional utility of stored and reconstituted peroxidase conjugates. In addition, it inhibits the conjugate from non-specifically binding to plate coating proteins or non-target sample proteins. By reducing non-specific conjugate bridging events on the plate surface, undesirable background signal-related losses in sensitivity can be all but eliminated.
Additional components may be added to HRP Conjugate Stock Stabilizer to optimize it for unique ELISA sample situations or for use in other immunological techniques.
- Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes.
- Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes.
- Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C, protected from light, providing a useful shelf-life of 6-12 months.
When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:
- To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer.
- Filter sterilize into a sterile amber or brown-glass container.
- Store at 2-8°C. Concentrated HRP conjugate stocks should be stable for up to 18 months.