Streptavidin Conjugated with Fluorescein (FITC)
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC).
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0.
Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band.
Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin;
Protease activity: 0.000018 units/mg
Isoelectric point: 7.0 +/- determined by Isoelectric focusing.
One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed.
This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies.
Avidin~Biotin conjugated reagents are stable, sensitive and give less background.
2-8 °C, in dark
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States