WGA-Biotin, Conjugated
This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction.
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction.
2-8 °C
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
Histochemistry 1:250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500.
Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions.
For Histochemistry: the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied, followed by streptavidin conjugated to enzyme and chromogen.
For WB: incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen.
For ELISA: the target proteins are absorbed on ELISA plate at a concentration of 5-10 µg/ml (50-100 µl), followed by blocking with protein solution, Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate.
Please refer to Histochemistry, WB and ELISA protocol for detail information.
Please refer to Histochemistry, WB and ELISA protocol for detail information.