ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.
![Green Fluorescent FAM-Leu-DAP Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/968-flisp_{width}x.jpg?v=1709768777)
![Green Fluorescent FAM-Leu-DAP Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/968-flisp-components_{width}x.jpg?v=1709768777)
![Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.](http://www.antibodiesinc.com/cdn/shop/files/960_figure_1_{width}x.jpg?v=1709768760)
![Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.](http://www.antibodiesinc.com/cdn/shop/files/960_figure_2_{width}x.jpg?v=1709768761)
![Red Fluorescent SR101-Leu-CMK Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/955-flisp_{width}x.jpg?v=1709768756)
![Red Fluorescent SR101-Leu-CMK Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/955-flisp-components_{width}x.jpg?v=1709768756)
![Red Fluorescent SR101-Phe-CMK Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/952-flisp_{width}x.jpg?v=1709768750)
![Red Fluorescent SR101-Phe-CMK Serine Protease Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/952-flisp-components_{width}x.jpg?v=1709768750)
![Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).](http://www.antibodiesinc.com/cdn/shop/files/949_figure_1_{width}x.jpg?v=1709768744)
![Figure 1. Jurkat cells were treated with staurosporine for 1, 2, 3, and 4 hours to induce apoptosis and increase serine protease activity (green), or were untreated (gray). Samples were stained with FAM-Phe-CMK (Kit 945/946) for 1 hour at 37°C, washed, and then analyzed using a flow cytometer. The amount of serine protease activity detected positively correlated to the duration of the exposure period. Data courtesy of Dr. Kristi Strandberg (ICT 231:75-79).](http://www.antibodiesinc.com/cdn/shop/files/945_figure_1_{width}x.jpg?v=1709768740)
![Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis and increase serine protease activity (upper), or left untreated (lower), then stained with FAM-Phe-CMK (Cat. 945/946), 660-VAD-FMK (cat. 9120), and Hoechst 33342. Imaged using EGFP (Ex 470/30, Em 530/50), Cy5 (Ex 620/60, Em 700/75), and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Staurosporine increased the intracellular levels of both serine proteases and caspases. Data courtesy of Dr. K. Strandberg (ICT 231:73).](http://www.antibodiesinc.com/cdn/shop/files/945_figure_2_{width}x.jpg?v=1709768739)
![Flow cytometry analysis of the caspase-10 activity in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.](http://www.antibodiesinc.com/cdn/shop/files/922-fc-1_{width}x.jpg?v=1730127525)
![Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/923-fam-flica-caspase-10_{width}x.jpg?v=1709768700)
![Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/918-fam-flica-caspase-2_{width}x.jpg?v=1709768693)
![Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit](http://www.antibodiesinc.com/cdn/shop/files/918-fam-flica-caspase-2-components_{width}x.jpg?v=1709768693)
![Figure 1. Activity of caspase-8 (using FAM-LETD-FMK) in AGS cells after 24 h incubation with Les-4367 (1 μM) and combined with anti-HER2 antibodies. From Figure 4 of A. Gornowicz et al Multi-Targeting Anticancer Activity of a New 4-Thiazolidinone Derivative with Anti-HER2 Antibodies in Human AGS Gastric Cancer Cells, Int J Mol Sci (2023) 24, 6791. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.](http://www.antibodiesinc.com/cdn/shop/files/99_figure_1_{width}x.png?v=1709768669)
![Flow cytometry analysis of the caspase-8 activity (Assay kit, Cat. no. 99) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.](http://www.antibodiesinc.com/cdn/shop/files/99-fc-1_{width}x.jpg?v=1730127036)
![Figure 1. Caspase activities in the microglia of white matter injury mice using our FAM-FLICA® kits. Only caspase 1 was significantly upregulated. Figure 1 (d) of L. He et al (2022) miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury, Hindaw Journal of Immunology Research 2022, 1642896. https://doi.org/10.1155/2022/1642896. Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 Liufang He et al.](http://www.antibodiesinc.com/cdn/shop/files/95_figure_1_{width}x.jpg?v=1709768661)