Fluorescent Total Cholesterol Detection Kit

A simple one step assay which can be used either as a Fluorometric or Colorimetric assay to detect the level of total cholesterol in samples. Cholesterol esters are hydrolyzed by cholesterol esterase. Cholesterol is detected via cholesterol oxidase.

Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.
Figure 2. Standard curve (absorbance). Absorbance read-out at 570 nm. R2 =0.9993. Incubation time = 30 minutes. Standard curve range 1.25μM to 80μM. A new curve must be generated each time the assay is run.
Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.
Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.
Figure 2. Standard curve (absorbance). Absorbance read-out at 570 nm. R2 =0.9993. Incubation time = 30 minutes. Standard curve range 1.25μM to 80μM. A new curve must be generated each time the assay is run.

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Data Sheet
Protocol
Safety Data Sheet

SKU: FLCHOL100-2

Size: 100 Tests
Price:
$357.00

Cholesterol is a lipid present in the cell membranes of eukaryotes and circulates in the blood stream. It is used in the biosynthesis of hormones, and plays an important role in cell signaling processes. Cholesterol exists as a free acid, as well as, in the esterified form as cholesteryl esters. Elevated levels of cholesterol are indicated in atherosclerosis and heart disease, and are the subject of large amount of research focused on cholesterol metabolism. Quantitative determination of cholesterol in experimental samples is central to this research.

This assay kit provides a simple, one-step fluorometric or colorimetric method for determination of total cholesterol in serum and plasma samples. The assay is based on an enzyme- coupled reaction that detects both free cholesterol and cholesterol esters. Cholesterol esters are hydrolyzed by cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase to yield hydrogen peroxide and cholest-4-en-3-one (ketone). The hydrogen peroxide then reacts with the cholesterol probe (detection reagent) in a 1:1 stoichiometry to produce the stable fluorescent product.

Absorbance or Fluorescence plate reader
Store contents as labeled
Ships overnight (domestic), International Priority Shipping
United States
  • 1. Cholesterol Probe (Part# 4022), Storage: -20°C
  • 2. Enzyme Mix (Part# 6023), Storage: -20°C
  • 3. 1x Reaction Buffer (Part# 3055), Storage 2-8°C
  • 4. Cholesterol Standard (Part# 7018), Storage: -20°C
  • 5. DMSO (Part# 7019), Storage 2-8°C

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