Fluoro NAD/NADH Detection Kit by Cell Technology

14 Citations

The kit uses a fluorescent readout to specifically detect NAD/NADH, with no reactivity with NADP/NADPH. It is suitable for use with cells and tissue from a range of sources such as animals, plants, bacteria and fungi.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).
Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.
Figure 3. The assay reacts with NAD and NADH. There is no reactivity with NADPH.
Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).
Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).
Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.
Figure 3. The assay reacts with NAD and NADH. There is no reactivity with NADPH.

Go to Full Product Details

Data Sheet
Protocol
Safety Data Sheet

SKU: FLNADH100-3

Size: 500 Tests
1-2 business days
Price:
$2,838.00

The role of pyridine nucleotides (NAD/NADH) metabolism in health is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation. These enzymes are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling, which suggests that either the enzymes or NAD could be an important therapeutical target.

The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.

The kit is suitable for applications such as:
- Detection of NAD/NADH activity in cells or tissue extracts.
- Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
- NAD/NADH detection in bacterial, fungal and plant cells.

530-570nm/590-600nm
Fluorescence plate reader
Store contents as labeled
Ships overnight (domestic), International Priority Shipping
United States
FLNADH100-2: 100 Tests
  • Part# 6021: Enzyme Mix x 1 (-20C)
  • Part# 4018: NADH Detection Reagent x 1 (-20C)
  • Part# 7013: NADH Standard x 1 (-20C)
  • Part# 3044: NAD/NADH Reaction Buffer x 1 (2-8 C)
  • Part# 3045: NAD/NADH Lysis Buffer x 1 (2-8 C)
  • Part# 3046: NAD Extraction Buffer x 1 (2-8 C)
  • Part# 3047: NADH Extraction Buffer x 1 (2-8 C)
  • Part# 3051: NADH Standard Diluent x 1 (2-8 C)

    • FLNADH100-3: 500 Tests
  • Part# 6021: Enzyme Mix x 5 (-20C)
  • Part# 4018: NADH Detection Reagent x 5 (-20C)
  • Part# 7013: NADH Standard x 5 (-20C)
  • Part# 3044: NAD/NADH Reaction Buffer x 5 (2-8 C)
  • Part# 3045: NAD/NADH Lysis Buffer x 5 (2-8 C)
  • Part# 3046: NAD Extraction Buffer x 5 (2-8 C)
  • Part# 3047: NADH Extraction Buffer x 5 (2-8 C)
  • Part# 3051: NADH Standard Diluent x 5 (2-8 C)

    • Product Specific References

    PMID Publication
    35504207Luan, Y., et al. 2022. STING modulates necrotic cell death in CD4 T cells via activation of PARP-1/PAR following acute systemic inflammation. nt Immunopharmacol, 108809.
    33593944Won, H.I., et al. 2021. Inactivation of the Pta-AckA Pathway Impairs Fitness of Bacillus anthracis during Overflow Metabolism. J Bacteriol, e00660-20.
    28299860Sadykov, M.R., et al. 2017. Poly(3-hydroxybutyrate) fuels the tricarboxylic acid cycle and de novo lipid biosynthesis during Bacillus anthracis sporulation . Mol Microbiol, 793-803.
    27374086Yin, C., et al. 2016. Exogenous pyruvate facilitates cancer cell adaptation to hypoxia by serving as an oxygen surrogate. Oncotarget, 47494-47510.
    27340697Gries, C.M., et al. 2016. Potassium Uptake Modulates Staphylococcus aureus Metabolism. mSphere, e00125-16.
    26322513Esteve-Gassent, M. D., et al. 2015. Absence of sodA Increases the Levels of Oxidation of Key Metabolic Determinants of Borrelia burgdorferi. PLoS One, e0136707.
    24670634Birsoy, K., et al. 2014. Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides. Nature, 108-12.
    23625849Sadykov, M.R., et al. 2013. Inactivation of the Pta-AckA pathway causes cell death in Staphylococcus aureus. J Bacteriol, 3035-44.
    23703906Wiley, S.E., et al. 2013. Wolfram Syndrome protein, Miner1, regulates sulphydryl redox status, the unfolded protein response, and Ca2+ homeostasis. EMBO Mol Med, 904-18.
    23703322Montero, J., et al. 2013. p53 regulates a non-apoptotic death induced by ROS. Cell Death Differ, 1465-74.
    23911327De Bock, K., et al. 2013. Role of PFKFB3-driven glycolysis in vessel sprouting . Cell, 651-63.
    22305999Martin, J. A., et al. 2012. Mitochondrial electron transport and glycolysis are coupled in articular cartilage. Osteoarthritis Cartilage, 323-9.
    22384140Schaue, D., et al. 2012. Cellular autofluorescence following ionizing radiation. PLoS One, e32062.
    19217932Bickler, P.E., et al. 2009. Inositol 1,4,5-triphosphate receptors and NAD(P)H mediate Ca2+ signaling required for hypoxic preconditioning of hippocampal neurons. Neuroscience, 51-60.

    Bulk Order Fluoro NAD/NADH Detection Kit by Cell Technology

    Related Products

    Recently Viewed