Highly Reactive Oxygen Species (hROS) Detection Kit

A general selective indicator for the detection of highly Reactive Oxygen Species (hROS), such as Hydroxyl Radical (•OH), Peroxynitrite(ONOO-), and hypochlorite (-OCl). It has little reactivity with other ROS.

Figure 1. Assay principle
Figure 2. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer and seeded in glass bottom dishes. The cells were then loaded with APF (10μM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters
Figure 1. Assay principle
Figure 1. Assay principle
Figure 2. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer and seeded in glass bottom dishes. The cells were then loaded with APF (10μM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters

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Data Sheet
Protocol

SKU: FLAPF100-2

Size: 150 Tests
1-2 business days
Price:
$289.00
Aminophenyl fluorescein (APF), developed by Tetsuo Nagano, is a general selective indicator for the detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS such as: singlet oxygen (O21), superoxide (O2– •), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•). APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (•OH), Peroxynitrite (ONOO-) and hypochlorite (-OCl) production in cells.
Fluorescence plate reader
2-8°C
Ships overnight (domestic), International Priority Shipping, NOT FOR SALE IN JAPAN.
United States
  • Part# 4011: APF Dye (2-8C)

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