Anti-c-FOS Antibody (2H2)

8 Citations

Our Anti-c-FOS primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat c-FOS and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Roll over image to zoom in Click on image to zoom
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunostaining of mouse brain labeling c-Fos protein in green(cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells (cat. 309-cFOS), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Immunolabeling of a section of rat brain that specifically labels c-FOS protein(cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
(A, B) confocal microscope images showing PV, WFA, and c-Fos (cat. 309-cFOS) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.
Immunofluorescence of young male and female wild type (WT) and Alzheimer's Disease (AD) mouse brain labelling nucleus-specific c-Fos + cells in the DMH, VMH, ARH, TuN, and LH regions. Image from publication CC-BY-4.0. PMID: 39272160

Bovine, Human, Mouse, Rat
ICC, IHC, WB
Mouse

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Data Sheet
Trial Size Trial Size

SKU: 309-cFOS

Volume: 100 µL
Price:
$445.00
Ships: 1-2 business days

Product Details

c-FOS
c-FOS is a member of the FOS transcription factor family which forms dimers with c-JUN to produce the Activator Protein 1 (AP-1) complex which plays a key role in critical cellular processes such as cell proliferation, differentiation and apoptosis (Chiu et al., 1988). c-FOS expression has been demonstrated to be a useful marker of neuronal activation as it is rapidly induced following various stimuli (Hoffman et al., 1993). Additionally, c-FOS has been shown to be overexpressed in a variety of malignant tumor types (Milde-Langosch 2005).
Protein G Purified
Monoclonal
2H2
IgG1
ICC, IHC, WB
Mouse
FOS
50 kDa
Recombinant full length human c-FOS expressed in and purified from E. Coli.
Bovine, Human, Mouse, Rat
AB_2632380
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
PBS + 50% glycerol and 5 mM NaN3
WB: 1:2000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Specific for endogenous levels of the ~50 kDa c-FOS protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Activator protein 1 antibody, AP 1 antibody, C FOS antibody, Cellular oncogene c fos antibody, Cellular oncogene fos antibody, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody, FBJ murine osteosarcoma viral oncogene homolog antibody, FBJ murine osteosarcoma viral v fos oncogene homolog antibody, FBJ Osteosarcoma Virus antibody, FOS antibody, FOS protein antibody, FOS_HUMAN antibody, G0 G1 switch regulatory protein 7 antibody, G0/G1 switch regulatory protein 7 antibody, G0S7 antibody, Oncogene FOS antibody, p55 antibody, proto oncogene c Fos antibody, Proto oncogene protein c fos antibody, Proto-oncogene c-Fos antibody, fos FBJ murine osteosarcoma viral oncogene homolog antibody
P01100
2353

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
392721601:250Qi, G., et al. 2024. Sex-specific hypothalamic neuropathology and glucose metabolism in an amyloidosis transgenic mouse model of Alzheimer's disease. Cell & Bioscience, 120.
36732588not listedBoutros, S.W., et al. 2023. Age, Sex, and Apolipoprotein E Isoform Alter Contextual Fear Learning, Neuronal Activation, and Baseline DNA Damage in the Hippocampus. Molecular Psychiatry, .
35468398 1:300 He, Z., et al. 2022. Genetic Deletion of TrpV1 and TrpA1 Does not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chemical Senses, 45(7), pp.573-579.
32572463 1:1000 Yu, T., et al. 2020. Genetic Deletion of TrpV1 and TrpA1 Does not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chemical Senses, 45(7), pp.573-579.
Immunohistochemistry: Rat
PMID Dilution Publication
395920891:1000Wallsten, B., et al. 2024. Response of parvalbumin interneurons and perineuronal nets in rat medial prefrontal cortex and lateral amygdala to stressor controllability. Brain Research, 149351.
393936831:1000Tzakis, N, et al. 2025. Assessment of cFos labeling in the hippocampus and anterior cingulate cortex following recent and remote re-exposure to an unreinforced open field in preadolescent and postadolescent rats. Behavioural Brain Research, 115284.
367781321:5000Maletta, T., et al. 2023. Preadolescent Exposure to a Sexually Mature, Unrelated Male Rat Reduces Postadolescent Social Recognition Memory and CA2 c-Fos Labeling. Frontiers in Behavioral Neuroscience, 1104866.
34650409 1:800 Ferraro, S., et al. 2021. In utero Exposure to Valproic-Acid Alters Circadian Organisation and Clock-Gene Expression: Implications for Autism Spectrum Disorders. Frontiers in Behavioral Neuroscience, 15.

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