Anti-GFP Antibody (N86/38)
Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/38. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/38 TC supe.
Product Specific References for Applications and Species
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Western Blot
- Whole Brain Imaging
- Unspecified
| Immunocytochemistry | ||
| PMID | Dilution | Publication |
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| 27621269 | not listed | Schwenk, B.M., et al. 2016. TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons. EMBO Journal, 2350-2370. |
| 26503721 | not listed | Thiffault, I., et al. 2015. A novel epileptic encephalopathy mutation in KCNB1 disrupts Kv2.1 ion selectivity, expression, and localization.. The Journal of General Physiology, 399-410. |
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| 25712101 | 1:200 | Gnerer, J.P., et al. 2015. Gene-specific cell labeling using MiMIC transposons.. Nucleic Acids Research, e56. |
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| 24729314 | 1:1000 | Nelson, M., et al. 2014. The sodium channel β1 subunit mediates outgrowth of neurite-like processes on breast cancer cells and promotes tumour growth and metastasis.. International Journal of Cancer, 2338-51. |
| 24667445 | 1:100 | Pathania, M., et al. 2014. The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines.. Translational Psychiatry, e374. |
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| 23808551 | 1:1000 | Gholizadeh, S., et al. 2013. Transduction of the central nervous system after intracerebroventricular injection of adeno-associated viral vectors in neonatal and juvenile mice.. Human Gene Therapy Methods, 205-13. |
| 23704356 | 1:200 | Asano, E., et al. 2013. The Aurora-B-mediated phosphorylation of SHCBP1 regulates cytokinetic furrow ingression.. Journal of Cell Science, 3263-70. |
| 23676497 | not listed | Marcello, E., et al. 2013. Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer''s disease.. Journal of Clinical Investigation, 2523-38. |
| 23094043 | 1:1000 | Mackenzie, S.M., et al. 2012. Proliferative regeneration of zebrafish lateral line hair cells after different ototoxic insults.. PLoS One, e47257. |
| 23094043 | 1:1000 | Mackenzie, S.M., et al. 2012. Proliferative regeneration of zebrafish lateral line hair cells after different ototoxic insults.. PLoS One, e47257. |
| 22545081 | 1:500 | Song, P. , et al. 2012. Knockdown of amyloid precursor protein in zebrafish causes defects in motor axon outgrowth.. PLoS One, e34209. |
| 22355657 | not listed | Ma, B., et al. 2012. Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons.. Scientific Reports, . |
| 21368030 | not listed | Malagelada, C., et al. 2011. RTP801/REDD1 regulates the timing of cortical neurogenesis and neuron migration.. Journal of Neuroscience, 3186-96. |
| 20823221 | not listed | Muir, J., et al. 2010. NMDA receptors regulate GABAA receptor lateral mobility and clustering at inhibitory synapses through serine 327 on the γ2 subunit. Procedings of the National Acadamy of Sciences, 16679-84. |
| 19837136 | 1:2000 | Ivanova, E., et al. 2010. Characterization of transgenic mouse lines expressing Cre recombinase in the retina.. Neuroscience, 233-43. |
| 25355692 | 1:200 | Wang, W.C., et al. 2015. Immunohistochemical localization of DPP10 in rat brain supports the existence of a Kv4/KChIP/DPPL ternary complex in neurons.. Journal of Comparative Neurology, 608-628. |
| Immunohistochemistry | ||
| PMID | Dilution | Publication |
| 31978073 | 1:200 | He, J., et al. 2020. Serotonin transporter dependent modulation of food-seeking behavior. PLoS One, e0227554. |
| 31504267 | 1:1000 | De Gregorio, R., et al. 2020. Disruption of Transient SERT Expression in Thalamic Glutamatergic Neurons Alters Trajectory of Postnatal Interneuron Development in the Mouse Cortex. Cerebral Cortex, 1623-1636. |
| 31006863 | 1:5000 | Éltes, T., et al. 2019. Improved spike inference accuracy by estimating the peak amplitude of unitary [Ca2+] transients in weakly GCaMP6f‐expressing hippocampal pyramidal cells. . The Journal of Physiology, 2925-2947. |
| 29634916 | 1:200 | Hinaux, H., et al. 2018. Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele. Deveopmental Biology, 111-123. |
| 29361569 | not listed | Wang, X., et al. 2018. Wnt6 maintains anterior escort cells as an integral component of the germline stem cell niche. Development, dev158527. |
| 27161034 | 1:100 | Battefeld, A., et al. 2016. Myelinating satellite oligodendrocytes are integrated in a glial syncytium constraining neuronal high-frequency activity. Nature Communications, 11298. |
| 26573957 | 1:200 | Ichinose, T., et al. 2015. Reward signal in a recurrent circuit drives appetitive long-term memory formation.. Elife, e10719. |
| 25632142 | 1:1000 | Firl, A., et al. 2015. Elucidating the role of AII amacrine cells in glutamatergic retinal waves.. Journal of Neuroscience, 1675-86. |
| 25337655 | 1:2000 | Kumar, D., et al. 2015. Specialized subpopulations of kisspeptin neurons communicate with GnRH neurons in female mice.. Endocrinology, 32-8. |
| 25042591 | 1:100 | Galili, D.S., et al. 2014. Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila.. Current Biology, 1712-22. |
| 24495972 | 1:200 | Davis, S.M., et al. 2014. Tailless and Atrophin control Drosophila aggression by regulating neuropeptide signalling in the pars intercerebralis.. Nature Communications, 3177. |
| 23867472 | 1:500 | Yin, Y., et al. 2013. Rapid induction of lung adenocarcinoma by fibroblast growth factor 9 signaling through FGF receptor 3.. Cancer Research, 5730-41. |
| Immunoprecipitation | ||
| PMID | Dilution | Publication |
| 34289359 | not listed | Li, L., et al. 2021. α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly. Cell Reports, 109396. |
| 30456350 | not listed | Hartmann, H., et al. 2018. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Science Alliance, e201800070. |
| 29217678 | not listed | Liu, P., et al. 2018. HRPU-2, a Homolog of Mammalian hnRNP U, Regulates Synaptic Transmission by Controlling the Expression of SLO-2 Potassium Channel in Caenorhabditis elegans. Journal of Neuroscience, 1073-1084. |
| 28388438 | not listed | Lee, J., et al. 2017. LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons. Molecular Cell, 129-140.e. |
| 27521566 | not listed | Hyodo, T., et al. 2016. A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1. FEBS Journal, 3662-3671. |
| 25684206 | not listed | Laurent, B., et al. 2015. A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation.. Molecular Cell, 957-970. |
| 25362473 | not listed | Ho, T.S.Y., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72. |
| 25362471 | not listed | Chang, K.J., et al. 2015. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81. |
| 25195858 | not listed | Santonico, E., et al. 2015. RNF11 is a GGA protein cargo and acts as a molecular adaptor for GGA3 ubiquitination mediated by Itch.. Oncogene, 3377-90. |
| 25486361 | not listed | Asano, E., et al. 2014. SHCBP1 is required for midbody organization and cytokinesis completion.. Cell Cycle., 2744-51. |
| 25059663 | not listed | Ikeuchi, Y., et al. 2014. TIF1γ protein regulates epithelial-mesenchymal transition by operating as a small ubiquitin-like modifier (SUMO) E3 ligase for the transcriptional regulator SnoN1.. The Journal of Biological Chemistry, 25067-78. |
| 24349395 | 1:500 | Parassol, N., et al. 2013. In vivo characterization of dynein-driven nanovectors using Drosophila oocytes.. PLoS One, e82908. |
| 22792260 | not listed | Thomas, A., et al. 2012. A versatile method for cell-specific profiling of translated mRNAs in Drosophila.. PLoS One, e40276. |
| 22665485 | not listed | Hyodo, T., et al. 2012. Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis.. The Journal of Biological Chemistry, 25019-29. |
| 21829588 | not listed | Roselli, F., et al. 2011. CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton.. PLoS One, e23097. |
| 20823221 | not listed | Muir, J., et al. 2010. NMDA receptors regulate GABAA receptor lateral mobility and clustering at inhibitory synapses through serine 327 on the γ2 subunit. Procedings of the National Acadamy of Sciences, 16679-84. |
| Western Blot | ||
| PMID | Dilution | Publication |
| 35104799 | 1:2000 | Wang, B., et al. 2022. Numb and Numblike regulate sarcomere assembly and maintenance. The Journal of clinical investigation, e139420. |
| 34728675 | not listed | Kang, S.U., et al. 2021. Integrative genome-wide analysis of dopaminergic neuron-specific PARIS expression in Drosophila dissects recognition of multiple PPAR-γ associated gene regulation. . Scientific reports, 1-14. |
| 31524157 | not listed | Rudenko, L.K., et al. 2019. Intraneuronal tau misfolding induced by extracellular amyloid-β oligomers. Journal of Alzheimer's Disease, 1125-1138. |
| 29118000 | not listed | Chowdhury, D., et al. 2018. Ca2+/calmodulin binding to PSD‐95 mediates homeostatic synaptic scaling down.. The EMBO journal, 122-138. |
| 29042443 | not listed | Shao, X., et al. 2017. Mammalian Numb protein antagonizes Notch by controlling postendocytic trafficking of the Notch ligand Delta-like 4.. Journal of Biological Chemistry, 20628-20643. |
| 26073430 | 1:1000 | Almeida, B., et al. 2015. SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97.. Biochimica et Biophysica Acta, 1950-9. |
| 25789526 | not listed | Chen, D., et al. 2015. EML4 promotes the loading of NUDC to the spindle for mitotic progression.. Cell Cycle, 1529-39. |
| 25450007 | not listed | Sato, N., et al. 2015. Inhibition of SNW1 association with spliceosomal proteins promotes apoptosis in breast cancer cells.. Cancer Med, 268-77. |
| 25362473 | not listed | Ho, T.S.Y., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72. |
| 24349395 | 1:500 | Parassol, N., et al. 2013. In vivo characterization of dynein-driven nanovectors using Drosophila oocytes.. PLoS One, e82908. |
| 24332851 | 1:1000 | Chen, D., et al. 2013. Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans.. Cell Report, 1600-10. |
| 23452848 | 1:2000 | Baker, S.A., et al. 2013. An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders.. Cell, 984-96. |
| 23444385 | 1:40 | Broekhuis, J.R., et al. 2013. SQL-1, homologue of the Golgi protein GMAP210, modulates intraflagellar transport in C. elegans.. Journal of Cell Science, 1785-95. |
| 23104136 | 1:2000 | Lu, V.B., et al. 2012. N-Arachidonyl glycine does not activate G protein-coupled receptor 18 signaling via canonical pathways.. Molecular Pharmacology, 267-82. |
| 23028782 | 1:1000 | Vapniarsky, N., et al. 2012. A lentiviral gene therapy strategy for the in vitro production of feline erythropoietin.. PLoS One, e45099. |
| 22792260 | not listed | Thomas, A., et al. 2012. A versatile method for cell-specific profiling of translated mRNAs in Drosophila.. PLoS One, e40276. |
| 22665485 | not listed | Hyodo, T., et al. 2012. Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis.. The Journal of Biological Chemistry, 25019-29. |
| 18676951 | not listed | Morishita, M. , et al. 2008. Sorting signals within the Saccharomyces cerevisiae sporulation-specific dityrosine transporter, Dtr1p, C terminus promote Golgi-to-prospore membrane transport.. Eukaryot Cell, 1674-84. |
| Whole Brain Imaging | ||
| PMID | Dilution | Publication |
| 31313987 | 1:1000 | Ki, Y. , et al. 2019. Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive. Elife, e40593. |
| Unspecified | ||
| PMID | Publication | |
| 32342988 | D'Orazi, F. D., et al. 2020. Conditional and biased regeneration of cone photoreceptor types in the zebrafish retina.. Journal of Comparative Neurology, 2816-2830. | |
| 26159098 | Morris, J.K., et al. 2015. Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio.. Invert Neuroscience, 4. | |
| 25451614 | Dissel, S., et al. 2015. Differential activation of immune factors in neurons and glia contribute to individual differences in resilience/vulnerability to sleep disruption.. Brain, Behavior, and Immunity, 75-85. | |
| 24962901 | Mandikian, D., et al. 2014. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors.. Journal of Comparative Neurology, 3555-74. | |
| 28007893 | Daria, A., et al. 2017. Young microglia restore amyloid plaque clearance of aged microglia.. The EMBO journal, 583–603. | |
| 27194325 | Antonelli, R., et al. 2016. Pin1 modulates the synaptic content of NMDA receptors via prolyl-isomerization of PSD-95.. Journal of Neuroscience, 5437-5447. | |
| 26573957 | Ichinose, T., et al. 2015. Reward signal in a recurrent circuit drives appetitive long-term memory formation.. Elife, e10719. | |
| 25926453 | Bosch, M.K., et al. 2015. Intracellular FGF14 (iFGF14) Is Required for Spontaneous and Evoked Firing in Cerebellar Purkinje Neurons and for Motor Coordination and Balance.. Journal of Neuroscience, 6752-69. | |
| 24394417 | Kline, C.F., et al. 2014. Ankyrin-B regulates Cav2. 1 and Cav2. 2 channel expression and targeting.. Journal of Biological Chemistry, 5285-5295. | |
| 24205342 | Slater, P.G., et al. 2013. Frizzled-5 receptor is involved in neuronal polarity and morphogenesis of hippocampal neurons.. PLoS One, e78892. | |
| 21525273 | Chen, X., et al. 2011. PSD-95 is required to sustain the molecular organization of the postsynaptic density.. Journal of Neuroscience, 6329-6338. | |