Our Anti-Alpha II Spectrin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Alpha II Spectrin and is Protein G purified. It is great for use in WB, IHC, ICC.
Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (cat. 98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (cat. 621-GFAP, 1:5000, red).
The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).
Protein G Purified
Monoclonal
3D7
IgG1
ICC, IHC, WB
Mouse
SPTAN1
240 kDa
Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin.
Bovine, Human, Mouse, Rat
AB_2492030
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specific for endogenous levels of the ~240 kDa alpha II spectrin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Bennett, V. and Baines, A.J., 2001. Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiological Reviews, 81(3), pp.1353-1392. PMID: 11427698
Eber, S. and Lux, S.E., 2004, April. Hereditary spherocytosis—defects in proteins that connect the membrane skeleton to the lipid bilayer. In Seminars in Hematology, 41(2),pp. 118-141. PMID: 15071790
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