Anti-βIII-Tubulin (C-terminus) Antibody

Name: Anti-βIII-Tubulin (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: TP1691
Price: 285.0 USD
Availability: InStock

4 Citations

Our βIII-Tubulin (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat βIII-Tubulin (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Immunocytochemical labeling in chick dorsal root ganglion neurons using anti-Cofilin (N-terminus; CP1131), anti-Cofilin (Ser-3; CP1151), anti-βIII-Tubulin (C-terminus; TP1691) and anti-β-Tubulin (TM1541) antibodies. (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).

Western blot analysis of mouse brain. The blot was probed with anti-unphosphorylated βIII-Tubulin (Ser-444) (lanes 1-3) and anti-βIII-Tubulin (C-terminus) (lanes 4-6) polyclonal antibodies. Both antibodies were used in the presence of unphosphorylated βIII-Tublin (Ser-444) peptide (lanes 2 & 5; TX1815) and phospho-βIII-Tublin (Ser-444) peptide (lanes 3 & 6; TX1695).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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SKU: TP1691

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

βIII-Tubulin (C-terminus)

Target Description

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

TUBB3

Molecular Weight

Antigen

βIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human βIII-Tubulin. This sequence is not found in bI or βII-Tubulin isotypes, but is well conserved in βIII-Tubulins from rat and mouse.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:2000
IHC: 1:500
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 50 kDa* protein corresponding to the molecular mass of βIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

TUBB3

UniProt ID

Q13509

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Human

Immunocytochemistry: Mouse
PMID	Dilution	Publication
25216639	1:500	Joyal, JS, et al. 2014. Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis. Nature medicine, 1165-1173.
23448719	not listed	Sapieha, P, et al. 2012. Omega-3 polyunsaturated fatty acids preserve retinal function in type 2 diabetic mice. Nutrition & Diabetes, e36.

Immunocytochemistry: Rat
PMID	Dilution	Publication
22108845	1:1000	Sheridan, GK, et al. 2012. S1P1 receptor subtype inhibits demyelination and regulates chemokine release in cerebellar slice cultures. Glia, 382-392.

Immunohistochemistry: Human
PMID	Dilution	Publication
29290996	not listed	Li, L, et al. 2017. The role of talin2 in breast cancer tumorigenesis and metastasis. Oncotarget, 106876-106887.

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Anti-βIII-Tubulin (C-terminus) Antibody

SKU: TP1691

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-βIII-Tubulin (C-terminus) Antibody

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