Our Anti-Calbindin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Calbindin and is Protein G purified. It is great for use in WB, IHC.
Immunofluorescence of a section of rat cerebellum labeled with anti-calbindin (cat. 302-CALB, 1:2,000, red), colabeled with anti-GFAP ( cat. 620-GFAP , 1:5000,green), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).
Protein G Purified
Monoclonal
IgG2a
IHC, WB
Mouse
CALB1
28 kDa
Recombinant full length human calbindin purified from E. coli.
Bovine, Human, Mouse, Rat
AB_2560939
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified.
PBS + 50% glycerol and 5 mM NaN3
WB: 1:2000
WB Brain: 1:2000
IHC: 1:1000-1:5000
Unconjugated
Specific for endogenous levels of the ~28 kDa calbindin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
avian-type antibody, CAB27 antibody, CALB 1 antibody, CALB antibody, CALB1 antibody, CALB1_HUMAN antibody, Calbindin 1 28kDa antibody, Calbindin antibody, Calbindin D28 antibody, D 28K antibody, D-28K antibody, D28K antibody, OTTHUMP00000166027 antibody, OTTHUMP00000225441 antibody, RTVL H protein antibody, Vitamin D dependent calcium binding protein antibody, Vitamin D dependent calcium binding protein avian type antibody, Vitamin D-dependent calcium-binding protein antibody
Andressen, C., Blümcke, I., & Celio, M. R. (1993). Calcium-binding proteins: selective markers of nerve cells. Cell and Tissue Research, 271(2), 181-208. PMID: 8453652
Kook, S.Y., Jeong, H., Kang, M.J., Park, R., Shin, H.J., Han, S.H., Son, S.M., Song, H., Baik, S.H., Moon, M. and Yi, E.C., 2014. Crucial role of calbindin-D 28k in the pathogenesis of Alzheimer’s disease mouse model. Cell Death and Differentiation, 21(10), p.1575. PMID: 24853300
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