Anti-ChAT (Choline Acetyltransferase) Antibody

Name: Anti-ChAT (Choline Acetyltransferase) Antibody
Brand: PhosphoSolutions
SKU: 315-ChAT
Price: 430.0 USD
Availability: InStock

2 Citations

Our Anti-ChAT (Choline Acetyltransferase) primary antibody from PhosphoSolutions is goat polyclonal. It detects chicken, guinea pig, human, mouse, and non-human primate, and rat ChAT (Choline Acetyltransferase) and is affinity purified. It is great for use in WB, IHC.

Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.

Species Reactivity

Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat

Applications

IHC, WB

Host Species

Goat

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Data Sheet

Trial Size

SKU: 315-ChAT

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

ChAT (Choline Acetyltransferase)

Target Description

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer's disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Format

Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Goat

Gene Name

CHAT

Molecular Weight

70 kDa

Antigen

Recombinant human choline acetyltransferase

Species Reactivity

Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2492055

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.

Buffer

PBS + 5 mg/ml BSA + 0.2% NaN3

Dilution Ranges

WB: 1:100
WB Brain: 1:100
IHC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~70 kDa choline acetyltransferase protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Acetyl CoA choline O acetyltransferase antibody, Acetyl CoA:choline O acetyltransferase antibody, ChAT antibody, CHOACTase antibody, Choline acetylase antibody, choline acetyltransferase antibody, Choline O acetyltransferase antibody, Choline O-acetyltransferase antibody, CLAT_HUMAN antibody, CMS1A antibody, CMS1A2 antibody, EC 2.3.1.6 antibody, OTTHUMP00000019583 antibody, OTTHUMP00000019584 antibody

UniProt Details

UniProt (Human): P28329
UniProt (Immunogen Species): P28329.

NCBI Gene ID

1103

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37508383	1:100	Hines, TJ, et al. 2023. A Novel ENU-Induced Mfn2 Mutation Causes Motor Deficits in Mice without Causing Peripheral Neuropathy. Biology, .

Immunohistochemistry: Mouse
PMID	Dilution	Publication
30640971	1:1000	Blandford, S., et al. 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative ophthalmology & visual science, 60(1), pp.183-191.

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-ChAT (Choline Acetyltransferase) Antibody

SKU: 315-ChAT

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-ChAT (Choline Acetyltransferase) Antibody

Product Specific Protocols

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