Anti-Coronin-1B (Ser-2), Phosphospecific Antibody

Name: Anti-Coronin-1B (Ser-2), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP2621
Price: 340.0 USD
Availability: InStock

3 Citations

Our Coronin-1B (Ser-2) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Coronin-1B (Ser-2) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of coronin-1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Coronin-1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2585 or CX2625).

Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Coronin-1B (C-terminal region) (lanes 1 & 2) or anti-Coronin-1B (Ser-2) (lanes 3 & 4).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Go to Full Product Details

SKU: CP2621

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

Coronin-1B (Ser-2)

Target Description

Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CORO1B

Molecular Weight

Antigen

Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-2

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Coronin2, Coro1B

UniProt Details

UniProt (Human): Q9BR76
UniProt (Immunogen Species): Q9BR76

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27592029	not listed	Kim, G.Y., et al. 2016. Coronin 1B serine 2 phosphorylation by p38α is critical for vascular endothelial growth factor-induced migration of human umbilical vein endothelial cells. Cellular Signalling, 1817-1825.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
22619279	not listed	Williams, H.C., et al. 2012. Role of coronin 1B in PDGF-induced migration of vascular smooth muscle cells. Circulation Research, 56-65.

Western Blot: Human
PMID	Dilution	Publication
27592029	not listed	Kim, G.Y., et al. 2016. Coronin 1B serine 2 phosphorylation by p38α is critical for vascular endothelial growth factor-induced migration of human umbilical vein endothelial cells. Cellular Signalling, 1817-1825.
24081143	not listed	Park, M., et al. 2013. Methamphetamine-induced occludin endocytosis is mediated by the Arp2/3 complex-regulated actin rearrangement. Journal of Biological Chemistry, 33324-33334.

Western Blot: Mouse
PMID	Dilution	Publication
22619279	not listed	Williams, H.C., et al. 2012. Role of coronin 1B in PDGF-induced migration of vascular smooth muscle cells. Circulation Research, 56-65.

Recover password

Create my account

Anti-Coronin-1B (Ser-2), Phosphospecific Antibody

SKU: CP2621

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Coronin-1B (Ser-2), Phosphospecific Antibody

Related Products

Recently Viewed