Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody (WB, E)

Name: Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: DP2241
Price: 340.0 USD
Availability: InStock

4 Citations

Our Dok1 (Tyr-362)[Dok2 (Tyr-337)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dok1 (Tyr-362)[Dok2 (Tyr-337)] and is antigen affinity purified. It is great for use in WB.

Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: DP2241

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

Dok1 (Tyr-362)[Dok2 (Tyr-337)]

Target Description

Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IκB Kinase β phosphorylates several serine sites including Ser-450 in vitro, and TNFα, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Clone

WB, E

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

DOK1

Molecular Weight

Antigen

Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment. Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-362

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

p62DOK

UniProt Details

UniProt (Human): Q99704
UniProt (Immunogen Species): Q99704

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
24762811	not listed	Barrett, A, et al. 2014. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. Journal of Cell Science, 2647-2658.

Western Blot: Mouse
PMID	Dilution	Publication
27926878	not listed	Davidson, D, et al. 2016. The Csk-Associated Adaptor PAG Inhibits Effector T Cell Activation in Cooperation with Phosphatase PTPN22 and Dok Adaptors. Cell reports, 2776-2788.
22826316	not listed	Gómez, CP, et al. 2012. The protein tyrosine phosphatase SHP-1 regulates phagolysosome biogenesis. Journal of Immunology (Baltimore, Md. : 1950), 2203-2210.
22736313	not listed	Besin, G, et al. 2012. Dok-1 overexpression promotes development of γδ natural killer T cells. European Journal of Immunology, 2491-2504.

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Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody (WB, E)

SKU: DP2241

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody

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