Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody

Name: Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP4101
Price: 340.0 USD
Availability: InStock

2 Citations

Our ERK1(T207)/ERK2(T188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus ERK1(T207)/ERK2(T188) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes 5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: EP4101

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

ERK1(T207)/ERK2(T188)

Target Description

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

MAPK1/2

Molecular Weight

42/44

Antigen

Phospho-ERK2 (Thr-188) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Thr-188 in mouse ERK2. This sequence is conserved in human, rat, chicken, and fish ERK2, and is highly conserved in ERK1 (Thr-207), ERK5 (Thr-224), and ERK7 (Thr-180).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects 42 and 44 kDa* proteins corresponding to ERK1 (Thr-207) and ERK2 (Thr-188) on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with calyculin A. It does not detect these ERK proteins in control cells or in blots treated with lambda phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-188

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ERK, p42, p44, MAPK

UniProt Details

UniProt (Human): P28482
UniProt (Immunogen Species): P63085

Product Specific References for Applications and Species

Western Blot: Human
Additional Publications: Unspecified

Western Blot: Human
PMID	Dilution	Publication
26249805	not listed	Huang, H, et al. 2015. ALK7 protects against pathological cardiac hypertrophy in mice. Cardiovascular Research, 50-61.

Additional Publications: Unspecified
PMID	Publication
23307144	Lu, J., et al. 2013. Interferon regulatory factor 3 is a negative regulator of pathological cardiac hypertrophy. Basic Research Cardiology, 326.

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Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody

SKU: EP4101

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody

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