Anti-GM130 (C-terminal region) Antibody (M342)

Name: Anti-GM130 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: GM3421
Price: 370.0 USD
Availability: InStock

6 Citations

Our GM130 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat GM130 (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.

Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: GM3421

Volume: 100 µL

Variant

Price:

$370.00

Quantity:

Product Details

Target

GM130 (C-terminal region)

Target Description

Golgi Matrix Protein (GM130) is a peripheral cytoplasmic protein that is bound to Golgi membranes. It maintains cis-Golgi structure, and it regulates the disassembly and reassembly of the Golgi complex during mitosis. GM130 may also be important during docking and fusion of coatomer (COPI) coated vesicles to the Golgi membrane. The carboxy-terminal domain of GM130 is highly homologous to the human auto-antigen, golgin-95. GM130 interacts in a GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes. It has also been implicated in the activation of Ste-kinase, YSK1, during the golgi reorganization that occurs along with cell migration.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M342

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

GOLGA2

Molecular Weight

130

Antigen

Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of GM130 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GOLGA2, golgi autoantigen

UniProt Details

UniProt (Human): Q08379
UniProt (Immunogen Species): Q08379

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Non-Human Primate | Porcine

Immunocytochemistry: Human
PMID	Dilution	Publication
29021346	1:50	Lin, Q., et al. 2017. The HECT E3 ubiquitin ligase NEDD4 interacts with and ubiquitylates SQSTM1 for inclusion body autophagy. Journal of Cell Science, 3839-3850.
28085563	not listed	Sun, A., et al. 2017. The E3 ubiquitin ligase NEDD4 is an LC3-interactive protein and regulates autophagy. Autophagy, 522-537.
25122799	not listed	Martín-Acebes, M.A., et al. 2014. The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis. Journal of Virology, 12041-12054.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37635877	not listed	Valdivia, A., et al. 2023. Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization. Frontiers in Cell and Developmental Biology, 1231489.

Immunocytochemistry: Non-Human Primate
PMID	Dilution	Publication
21949814	not listed	Martín-Acebes, M.A., et al. 2011. West Nile virus replication requires fatty acid synthesis but is independent on phosphatidylinositol-4-phosphate lipids. PLoS One, e24970.

Immunocytochemistry: Porcine
PMID	Dilution	Publication
27199941	not listed	Vázquez-Calvo, Á., et al. 2016. The Amino Acid Substitution Q65H in the 2C Protein of Swine Vesicular Disease Virus Confers Resistance to Golgi Disrupting Drugs. Frontiers in Microbiology, 612.

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Anti-GM130 (C-terminal region) Antibody (M342)

SKU: GM3421

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-GM130 (C-terminal region) Antibody

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