Our Histone H2B (Ser-36) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Histone H2B (Ser-36) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).
The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of each histone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generate chromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translational modification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibit transcription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine the chromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode of regulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylation include Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increased transcription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reduces transcription of AMPK-dependent genes and lowers cell survival.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
H2BC3
15
Phospho-Histone H2B (Ser-36) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-36 in human histone H2B. This sequence is well conserved in most isoforms of rat and mouse histone H2B, and has low homology to other histone family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
Unconjugated
The antibody detects a 15 kDa* protein corresponding to the molecular mass of Histone H2B on SDS-PAGE immunoblots of human Jurkat cells treated with Calyculin A, and this reactivity is reduced after lambda phosphatase treatment.
Phosphorylated
Ser-36
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Li, J., et al. 2016. Nuclear PKC-θ facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. Journal of Cell Science, 2448-2461.
Lee, J.H., et al. 2015. AKT phosphorylates H3-threonine 45 to facilitate termination of gene transcription in response to DNA damage. Nucleic Acids Research, 4505-4516.
Soe, K.C., et al. 2013. Transcriptional coactivator CIITA, a functional homolog of TAF1, has kinase activity. Biochimica et Biophysica Acta, 1184-1190.
Nam, K.H., et al. 2018. Eudesmin impairs adipogenic differentiation via inhibition of S6K1 signaling pathway. Biochemical and Biophysical Research Communications, 1148-1153.
Bertoldo, M.J., et al. 2016. Specific deletion of AMP-activated protein kinase (α1AMPK) in mouse Sertoli cells modifies germ cell quality. Molecular and Cellular Endocrinology, 96-112.
