Anti-IκBα (Tyr-305), Phosphospecific Antibody

Name: Anti-IκBα (Tyr-305), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: IP1041
Price: 340.0 USD
Availability: InStock

2 Citations

Our IκBα (Tyr-305) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat IκBα (Tyr-305) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IκBα (lane 1), anti-IκBα (Tyr-42) (IP1031; lanes 2-5), or anti-IκBα (Tyr-305) (IP1041; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IκBα (Tyr-42) (lane 3 & 8) or IκBα (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: IP1041

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

IκBα (Tyr-305)

Target Description

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

NFKBIA

Molecular Weight

Antigen

IκBα (Tyr-305) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 305 of human IκBα. This peptide sequence has low homology to other IκB proteins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IκBα (Tyr-305) peptide (without carrier). The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but does not detect this band in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-305

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa

UniProt Details

UniProt (Human): P25963
UniProt (Immunogen Species): P25963

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
30887112	not listed	Pontoriero, M, et al. 2019. Activation of NF-κB in B cell receptor signaling through Bruton's tyrosine kinase-dependent phosphorylation of IκB-α. Journal of molecular medicine (Berlin, Germany), 675-690.
25671697	not listed	Cullen, S., et al. 2015. Redox-regulated pathway of tyrosine phosphorylation underlies NF-κB induction by an atypical pathway independent of the 26S proteasome. Biomolecules, 95-112.

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Anti-IκBα (Tyr-305), Phosphospecific Antibody

SKU: IP1041

Product Details

Product Specific References for Applications and Species

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