Anti-Mitochondria Antibody (113-1)

Name: Anti-Mitochondria Antibody
Brand: PhosphoSolutions
SKU: 1155-MIT
Price: 375.0 USD
Availability: InStock

Our Anti-Mitochondria primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Mitochondria and is Protein A purified. It is great for use in WB, IHC, ICC.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa mitochondrial protein.

Immunofluorescence of HeLa cells labeled with anti-mitochondria antibody (1:100, green).

Species Reactivity

Human

Applications

IF, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1155-MIT

Volume: 100 µL

Variant

Price:

$375.00

Quantity:

Product Details

Target

Mitochondria

Target Description

Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Format

Protein A Purified

Clonality

Monoclonal

Clone

113-1

Isotype

IgG1

Applications

IF, IHC, WB

Host Species

Mouse

Molecular Weight

60 kDa

Antigen

Human cell homogenate

Species Reactivity

Human

Antibody Registry ID

AB_2492154

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein A purified culture supernatant

Buffer

PBS

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:800
ICC: 1:100-1:800

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

mitochondrial marker

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Product Specific Protocols

Western Blot Protocol: Download

General References

McBride HM, Neuspiel M, Wasiak S (2006) Mitochondria: More than just a powerhouse. Curr Biol., 16(14):R551-60. PMID: 16860735
Green DR (1998) Apoptotic pathways: the roads to ruin. Cell, 94(6):695-8. PMID: 9753316

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Anti-Mitochondria Antibody (113-1)

SKU: 1155-MIT

Product Details

Bulk Order Anti-Mitochondria Antibody

Product Specific Protocols

General References

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