Our Anti-Munc-18 (Ser 513) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Munc-18 (Ser 513) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser513 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996). Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
STXBP2
65 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:400
Unconjugated
Specific for endogenous levels of the ~65 kDa Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser513
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
