Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

Name: Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: MP3791
Price: 275.0 USD
Availability: InStock

3 Citations

Our Myosin IIA Heavy Chain (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin IIA Heavy Chain (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).

Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: MP3791

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

Myosin IIA Heavy Chain (C-terminal region)

Target Description

Nonmuscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MYH9

Molecular Weight

200

Antigen

NMHC-IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:2000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 200 kDa* protein corresponding to the apparent molecular mass of NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

NMHC-IIA, MYH9, myosin heavy chain

UniProt Details

UniProt (Human): P35579
UniProt (Immunogen Species): P35579

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37218133	1:2000	Vitriol, EA, et al. 2023. Nonmuscle Myosin 2 Filaments are Processive in Cells. Biophysical Journal, .

Western Blot: Mouse
PMID	Dilution	Publication
37218133	1:2000	Vitriol, EA, et al. 2023. Nonmuscle Myosin 2 Filaments are Processive in Cells. Biophysical Journal, .
27020697	1ug/ml	Stritt, S, et al. 2016. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nature communications, 11097.

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Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

SKU: MP3791

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

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