Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody

Name: Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP3411
Price: 340.0 USD
Availability: InStock

3 Citations

Our p38α MAP Kinase (Tyr-323) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat p38α MAP Kinase (Tyr-323) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38α (a.a. 319-328) (lanes 1 & 2) or anti-p38α (Tyr-323) (lanes 3 & 4).

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: PP3411

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

p38α MAP Kinase (Tyr-323)

Target Description

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

Mapk14

Molecular Weight

Antigen

Phospho-p38α MAP Kinase (Tyr-323) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 323 in mouse p38α. This peptide sequence is highly conserved in human and rat p38α, and has high homology to the conserved site in p38β.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:300

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to a phospho-tyrosine peptide before affinity purification using phospho-p38α (Tyr-323) peptide (without carrier). The antibody detects a 38 kDa* band corresponding to p38α on SDS-PAGE immunoblots of human Jurkat, K562, and mouse macrophage (J774A.1) cells treated with pervanadate. In addition, the antibody detects recombinant p38 MAP Kinase when treated with Fyn kinase (The in vitro kinase model was kindly provided by Dr. Paul Mittelstadt from the Laboratory of Immune Cell Biology at the NCI.)

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-323

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MAPK, p38, p38alpha, p38MAPK

UniProt Details

UniProt (Human): Q16539
UniProt (Immunogen Species): P47811

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Mouse
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
34960191	not listed	Guha, J, et al. 2021. Disc Large Homolog 1 Is Critical for Early T Cell Receptor Micro Cluster Formation and Activation in Human T Cells. Vaccines, 1446.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26479921	not listed	Alam, M.S., et al. 2015. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression. Nature Medicine, 1337-1343.

Western Blot: Human
PMID	Dilution	Publication
34960191	not listed	Guha, J, et al. 2021. Disc Large Homolog 1 Is Critical for Early T Cell Receptor Micro Cluster Formation and Activation in Human T Cells. Vaccines, 1446.
25151490	not listed	Lanna, A., et al. 2014. The kinase p38 activated by the metabolic regulator AMPK and scaffold TAB1 drives the senescence of human T cells. Nature Immunology, 965-972.

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Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody

SKU: PP3411

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody

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