Anti-Paxillin Antibody (M107)

Name: Anti-Paxillin Antibody
Brand: PhosphoSolutions
SKU: PM1071
Price: 370.0 USD
Availability: InStock

4 Citations

Our Paxillin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Paxillin and is Protein A purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: PM1071

Volume: 100 µL

Variant

Price:

$370.00

Quantity:

Product Details

Target

Paxillin

Target Description

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration

Format

Protein A Purified

Clonality

Monoclonal

Clone

M107

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

PXN

Molecular Weight

Antigen

Clone (M107) was generated from full-length recombinant human paxillin.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P49023
UniProt (Immunogen Species): P49023

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Western Blot: Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
31615975	1:500	Cho, MJ, et al. 2019. Oxidative stress-mediated TXNIP loss causes RPE dysfunction. Experimental & molecular medicine, 45304.

Immunocytochemistry: Rat
PMID	Dilution	Publication
17978823	not listed	Davis, R.C., et al. 2008. A cell culture model for investigation of Hirano bodies. Acto Neuropathology, 205-217.

Western Blot: Mouse
PMID	Dilution	Publication
34168556	1:2500	Fan, Y, et al. 2021. Salidroside Improves Chronic Stress Induced Depressive Symptoms Through Microglial Activation Suppression. Frontiers in pharmacology, 635762.
25107909	not listed	Lee, I.C.J., et al. 2014. Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics. Journal of Biological Chemistry, 26989-27003.

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Anti-Paxillin Antibody (M107)

SKU: PM1071

Product Details

Product Specific References for Applications and Species

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