Our PTP1B (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat PTP1B (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).
PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
PTPN1
50
PTP1B synthetic peptide (coupled to carrier protein) corresponds to amino acids from the N-terminal region of human PTP1B. This sequence has significant homology to the conserved site in rat and mouse PTP1B, and has homology to TC-PTP and MPTP, but not other protein tyrosine phosphatase family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
The antibody detects a 50 kDa* band corresponding to PTP1B in western blots of human A431, Jurkat, HeLa, and mouse SYF cells. This reactivity can be specifically blocked using PTP1B (a.a. 146-157) peptide (PX2355).
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
